TÜRKÇE
ENGLISH
Information
| Code | BT505 |
| Name | Basic Principles of Gene Cloning |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BURHAN ARIKAN |
| Course Instructor |
1 |
Course Goal / Objective
Gene Cloning and its applications
Course Content
The Importance of Gene Cloning, Properties and Functions of Plasmid and Bacteriophages, Isolation and Purification of Chromosomal, Pazmid and Bacteriophage DNAs from Live Organisms, Assignment of DNA Using Transformational Systems to Live Organisms and the Properties of Techniques Used in Cloning. Polymerase chain reaction, Microbiology, cloning in agriculture and medicine
Course Precondition
none
Resources
Gene cloning and DNA analyses
Notes
Gene applications
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | To be able to evaluate the knowledge and skills acquired in the field with a critical approach and to direct the learning |
| LO02 | To be able to transfer the current developments and his / her studies to the groups in the field and outside of the field by supporting them with quantitative and qualitative data systematically. |
| LO03 | To be able to follow international studies related to the field, to take part in various social interaction and scientific working groups at international level and to have communication skills in at least one foreign language in order to share their studies at international level |
| LO04 | To be able to use computer software and information and communication technologies at a sufficient level required by the field of biotechnology |
| LO05 | Ability to use the knowledge, problem solving and / or application skills in interdisciplinary studies |
| LO06 | To be able to deepen the general knowledge of biotechnology science within the framework of basic theories and applications in the field of study |
| LO07 | Being aware of social, scientific and ethical values in the studies conducted in the field; to carry out the research process within the framework of these values |
| LO08 | To be able to critically evaluate assumptions, thoughts and theories related to biotechnology with a skeptical, logical, analytical and independent perspective. |
| LO09 | Comprehensive knowledge about modern techniques and methods applied in natural and applied sciences and their boundaries |
| LO10 | To be able to develop and deepen knowledge in the field of biotechnology |
| LO11 | To be able to comprehend the interdisciplinary interaction related to biotechnology |
| LO12 | Ability to interpret and integrate the knowledge gained in biotechnology with information from different disciplines and to create new knowledge |
| LO13 | To be able to solve problems encountered in the field by using research methods |
| LO14 | To be able to conduct an independent study related to the field |
| LO15 | To be able to develop new strategic approaches to solve unforeseen complex problems encountered in applications related to biotechnology and to be able to produce solutions by taking responsibility |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 2 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 4 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 2 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Importance of Gene Cloning, | Study related topics | Öğretim Yöntemleri: Anlatım |
| 2 | Plasmide | Study related topics | Öğretim Yöntemleri: Anlatım |
| 3 | Bakteriophage | Study related topics | Öğretim Yöntemleri: Anlatım |
| 4 | Chromosomal DNA, plasmid and bacteriophage isolation techniques from bacteria | Study related topics | Öğretim Yöntemleri: Anlatım, Beyin Fırtınası |
| 5 | Chromosomal DNA isolation techniques from plants | Study related topics | Öğretim Yöntemleri: Anlatım |
| 6 | Chromosomal DNA isolation methods from yeast | Study related topics | Öğretim Yöntemleri: Anlatım |
| 7 | Chromosomal DNA isolation methods from fungi | Study related topics | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Ödev |
|
| 9 | Conjugation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 10 | Transformation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 11 | Electroporation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 12 | Electrofusion, biolistic transformation and liposome transformation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 13 | Cloning vectors | Study related topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Recombinant DNA production and transfer to the recipient organism | Study related topics | Öğretim Yöntemleri: Anlatım, Tartışma |
| 15 | Polymerase chain reaction and applications | Study related topics | Öğretim Yöntemleri: Anlatım, Tartışma, Gösterip Yaptırma |
| 16 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav, Ödev, Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav, Ödev, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||