TÜRKÇE
ENGLISH
Information
| Code | BT075 |
| Name | Laboratory Applications in Animal Biotechnology |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BAHRİ DEVRİM ÖZCAN |
| Course Instructor |
1 |
Course Goal / Objective
Transfer of the basic techniques used in the field of biotechnology as theoretical and practical to postgraduate students
Course Content
Considerations in laboratory studies, solution preparation, DNA and RNA isolation from procaryotes and eukaryotes, electrophoresis, purification of DNA from agarose, isolation and electrophoresis of proteins, PCR technology
Course Precondition
No prerequisite
Resources
Animal Biotechnology, 2018. A.K. Srivastava, R.K. Singh, M.P. Yodav, CBS PUB & DIST PVT LTD INDIA, ISBN-13 : 978-8120416482
Notes
Advances in Animal Biotechnology, 2019. B. Singh, G. Mal, S.K. Gautam, M. Mukesh, Springer, ISBN: 978-3-030-21309-1
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Use tools and equipment that can be found in a biotechnology laboratory |
| LO02 | Prepare the media and solutions that can be used in laboratory |
| LO03 | Isolate DNA and RNA from prokaryotic and eukaryotic organisms |
| LO04 | Performs electrophoretic analysis of nucleic acid and protein molecules |
| LO05 | Amplify a specific region on the DNA molecule using the PCR technique |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 2 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 2 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 2 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General introduction of biotechnology laboratory and, rules to be followed | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Bacterial media and solution preparation | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Genomic DNA isolatipon from bacteria | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Plasmid DNA isolation from isolation | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Determination of quantity and quality of DNA molecules | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Agarose gel electrophoresis | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Protein isolation from bacteria | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | SDS-PAGE | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 10 | DNA isolation from eukaryotic tissues | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | PCR and PCR types | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım |
| 12 | DNA purification from agarose gel | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | Nucleic acid-modifying enzymes and their use in biotechnology | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım |
| 14 | Hybridization methods 1 | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım |
| 15 | Hybridization methods 2 | All biotechnology and molecular biology laboratory books | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Term exams | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Term exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||