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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| PLANT PROTECTION (MASTER) (WITH THESIS) | |
| Code | BK049 |
| Name | Application of Biotechnological Methods on Fungi |
| Term | 2025-2026 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUKADDES KAYIM |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
he objective is to teach biotechnological methods in plant pathogenic fungi. For this purpose, it includes amplifying genes used in molecular diagnostics through PCR-based techniques, performing nucleotide analyses to construct phylogenetic trees, and comparing the results with both molecular and traditional methods (morphological, cultural, and physiological).
Course Content
ntroduction to the Fungal Kingdom, its Classification, Molecular Characterization of Fungi Purified from Diseased Plant Tissues, Phylogenetic Analysis, Classification Based on Gene Groups, Gene Transfers in Fungi, and the Application Areas and Methods of Biotechnological Techniques
Course Precondition
plant Fungi Disease
Resources
Funguslara Giriş (John Webster and Roland Weber, 2007 Cambridge University Yayınları); Modern Mikoloji (J.W. Deacon, 2001, 3. baskı)
Notes
Lecture notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns how to isolate and purify pathogenic fungi from diseased plants and infested soil. Gains knowledge of microscopy techniques used for studying fungi. |
| LO02 | Learns nucleic acid isolation techniques from fungal cultures. Gains knowledge about the characteristics of genes involved in fungal identification. |
| LO03 | Learns how to amplify diagnostically important genes using PCR-based methods. Gains skills in comparing and matching the amplified genes with sequences in genetic databases. |
| LO04 | Learns to compare classical and molecular methods for the accurate identification and diagnosis of plant pathogenic fungi. |
| LO05 | Learns how genetically modified transgenic plants can be used in the control of plant pathogenic fungi. |
| LO06 | Understands the importance of molecular methods in the classification of fungi. Learns the cultural and physical characteristics of fungi. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Has knowledge on basic topics about general science, general agricultural engineering and plant production | 1 |
| PLO02 | Yetkinlikler - Öğrenme Yetkinliği | Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants | 3 |
| PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice | |
| PLO04 | Yetkinlikler - Öğrenme Yetkinliği | Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can present results of the scientific research as a report/thesis and produce scientific publications | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develops skills in using laboratory methods and techniques | |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General Characteristics of Plant Pathogenic Fungi: | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Classical Classification of Plant Pathogenic Fungi | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri |
| 3 | An overview of molecular methods used in the classification of plant pathogenic fungi. | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösteri |
| 4 | Roles of Genes Used in the Classification of Plant Pathogenic Fungi | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösterip Yaptırma |
| 5 | Isolation and Purification Methods of Plant Pathogenic Fungi from Diseased Plant Tissues | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 6 | Determination of Cultural and Morphological Characteristics of Purified Fungi | Lecture Notes and References | Öğretim Yöntemleri: Gösterip Yaptırma, Anlatım |
| 7 | DNA Isolation Methods from Pure Fungal Cultures and PCR Techniques for DNA Amplification | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösterip Yaptırma, Gösteri |
| 8 | Mid-Term Exam | Written Exam | Ölçme Yöntemleri: Ödev, Yazılı Sınav |
| 9 | PCR Amplification Methods of Internal Transcribed Spacer (ITS), Elongation Factor 1, Beta Tubulin (TUB2), SSU, LSU, Actin (ACT), Calmodulin (CAL), Chitin Synthase (CHS-1), Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), and Glutamine Synthase (GS) Genes | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 10 | Comparative Analysis of Gene Sequences in Fungal Identification and Classification | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 11 | Integration of Cultural and Morphological Methods with Molecular Techniques in Fungal Identification and Classification | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 12 | Phylogenetic Analysis of Genes – Part 1 (According to Fungal Divisions) | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 13 | Classification Based on Phylogenetic Analysis I (with Examples) | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 14 | Phylogenetic Tree Construction Methods for Fungal Classification II | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 15 | Developing Fungal-Resistant Plants through Molecular and Biotechnological Methods – GMOs | Lecture Notes and References | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 16 | Term Exams | Written Exam | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 17 | Term Exams | Written Exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||