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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| MEDICAL BIOCHEMISTRY (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | BİK571 |
| Name | ATOMIC ABSORBTION SPECTROPHOTOMETER |
| Term | 2025-2026 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The objective of this course is to provide students with a deep understanding of atomic absorption spectroscopy (AAS) and enable them to apply this technique for the analysis of elements in biological, environmental, and industrial samples. Students will learn the theoretical principles of atomic absorption, the structure and operation of AAS instruments, and how to perform practical analyses in a laboratory
Course Content
Basics of AAS: What is atomic absorption spectroscopy and how does it work? Instrument Structure and Operating Principles: AAS devices, atomic source, detectors, monochromators. Atomization Techniques: Lamp atomic source, graphite furnace atomization, electrothermal atomization. Application Areas: Metal analysis, applications in biological and environmental samples. Data Analysis and Calibration: Standard curve construction, internal and external standard usage.
Course Precondition
Biochemistry and Analytical Chemistry: Basic knowledge of chemistry and biochemistry, including sample analysis and principles of spectroscopic techniques. Spectroscopic Methods: Knowledge of UV-Vis spectroscopy and basic photometry. Chemistry and Physical Chemistry: Understanding of chemical reactions, optical properties, and the theory of photometry.
Resources
lippincott
Notes
"Atomic Absorption Spectrometry" - David A. Skoog, Donald M. West, F. James Holler"Principles of Instrumental Analysis" - Douglas A. Skoog, F. James Holler, Timothy A. Nieman
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Understand the Basics of Atomic Absorption Spectroscopy: |
| LO02 | Operate AAS Instruments: |
| LO03 | Select Different Atomization Techniques: |
| LO04 | Learn Sample Preparation and Analysis Methods: |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Explain the structure and function of the basic tissues of the body. | 4 |
| PLO02 | Belirsiz | Explain the structure and functions of the cell, their physiological mechanisms and bioelectrical events. | 3 |
| PLO03 | Belirsiz | Explain the structure and functions of biochemical molecules involved in metabolism. | 5 |
| PLO04 | Belirsiz | Know the structure and function of genetic material (DNA and RNA), gene expression, genome structure, nucleic acid metabolism and the use of DNA in medicine, the basic principles of heredity. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Course Introduction and Fundamentals of Atomic Absorption Spectroscopy | Review basic concepts of AAS and spectroscopic techniques. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 2 | AAS Instruments and Operating Principles | Learn about the general structure of AAS instruments and explore visual aids. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 3 | Analytical Methods and Applications | Investigate the use of AAS for metal analysis and research biological sample analysis. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 4 | Atomization Techniques: Lamp Atomic Source | Read papers about lamp atomic source and how atomization works. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 5 | Atomization Techniques: Graphite Furnace Atomization | tudy graphite furnace atomization and perform a comparative analysis with other techniques. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 6 | Atomization Techniques: Electrothermal Atomization | xplore differences between electrothermal atomization and other methods. | Öğretim Yöntemleri: Tartışma, Anlatım |
| 7 | Sample Preparation and Matrix Effects | Review literature on sample preparation and matrix effects. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Standard Curve and Calibration Methods | ead about standard curve creation and calibration techniques. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 10 | Sensitivity and Resolution | Study examples of sensitivity and resolution measurements in spectroscopic analysis. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 11 | Integrated Instruments and AAS with Other Techniques | Research about integrated devices and the applications of AAS combined with other techniques. | Öğretim Yöntemleri: Tartışma, Anlatım |
| 12 | Spectrophotometric Methods and Comparison with AAS | Compare UV-Vis spectroscopy and AAS, explore their differences. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 13 | Optical Coherence and AAS | nvestigate the impact of optical coherence on AAS. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 14 | Metal Analysis in Biological Samples | Review research papers about metal analysis in biological samples and practice related case studies. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 15 | Industrial and Environmental Applications | Conduct research on industrial and environmental applications of AAS. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||