Information
| Unit | FACULTY OF AGRICULTURE |
| GARDEN PLANTS PR. | |
| Code | BBP352 |
| Name | Molecular Breeding Methods in Horticulture |
| Term | 2017-2018 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. SALİH KAFKAS |
| Course Instructor |
Prof. Dr. SALİH KAFKAS
(Bahar)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
Defination and applications of biotechnology, the use of biotechnology in plant breeding
Course Content
This lesson contains the definition and application areas of biotechnology, tissue culture laboratory organization, embryo rescue and embryo culture, somatic embryogenesis and organogenesis, obtaining haploid plants, protoplast culture and somatic hybridization, plants, gene transfer methods, molecular genetics laboratory organization, DNA, RNA structures Investigation, PCR mechanism genetic markers: morphological and biochemical markers, molecular marker technology and GMOs, and the situation of the world.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Compare DNA and RNA in terms of similarities and differences. |
| LO02 | Defines molecular marker techniques. |
| LO03 | Defines and tests Polymerase Chain Reaction. |
| LO04 | Explains the methods of electrophoresis |
| LO05 | Memorises molecular breeding methods based on markers in horticultural plant breeding. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Adequate knowledge on subjects specific to the discipline of Mathematics, Science and Agricultural Engineering (Horticulture), ability to use theoretical and applied knowledge in these fields in complex engineering problems | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | The ability to identify and solve problems related to the cultivation, breeding and product preservation of fruit, vegetables, vineyards and ornamental plants in horticulture, the ability to choose and apply appropriate analysis and modeling methods for this purpose. | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | The ability to design in a way that meets the necessary conditions for the cultivation of fruit, vegetables, vineyards and ornamental plants in the open and greenhouse in horticulture and the ability to apply modern design methods for this purpose. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Ability to select and use modern tools necessary for the analysis and solution of complex problems encountered in horticulture practices, ability to use information technologies effectively | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Ability to design and conduct experiments, collect data, analyze and interpret results for the study of complex problems or discipline-specific research issues in the field of Horticulture | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Breeding of Horticultural Plants, developing new varieties, making selection, protecting genetic resources, producing propagation materials (seeds, seedlings, saplings) of developed varieties, ability to work in individual and multi-disciplinary teams | 3 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Ability to write effective reports in the field of Horticulture, to understand written reports, to prepare design and production reports, to make effective presentations, to take and give clear and understandable instructions | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Awareness of the necessity of lifelong learning, the ability to access information in the field of Horticulture, to follow the developments in science and technology and to constantly renew oneself | 1 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Behaving in accordance with ethical principles, professional and ethical responsibility in the field of Horticulture, and knowledge of standards used in engineering practices | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Information about applications in business life, such as project management, risk management and change management in the field of Horticulture, awareness of entrepreneurship, innovation, information about sustainable development | |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Knowledge of the effects of horticultural practices on health, environment and safety in universal and social dimensions and the problems of the age reflected in the field of Horticulture, awareness of the legal consequences of horticultural solutions |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA and RNA structure | Reading of the papers about the lecture. | |
| 2 | DNA and RNA isolation and methods used for determination the amount of DNA and RNA | Reading of the papers about the lecture. | |
| 3 | The genetic markers and the history | Reading of the papers about the lecture. | |
| 4 | PCR technologies and the use area | Reading of the papers about the lecture. | |
| 5 | Electrophoresis systems | Reading of the papers about the lecture. | |
| 6 | Molecular marker technologies and the use in the plant breeding | Reading of the papers about the lecture. | |
| 7 | PCR-based molecular markers | Reading of the papers about the lecture. | |
| 8 | Determination of the genetic relationships of plants using molecular markers and the use of genetic similarities inplant breeding | Reading of the papers about the lecture. | |
| 9 | The creation of genetic maps | Reading of the papers about the lecture. | |
| 10 | Mid-Term Exam. | Preparation to mid-term exam. | |
| 11 | The use of genetic maps in plants breeding | Reading of the papers about the lecture. | |
| 12 | Marker assistant selection for plant breeding | Reading of the papers about the lecture. | |
| 13 | Molecular methods used in determination gene expression in plant and the use in plant breeding | Reading of the papers about the lecture. | |
| 14 | The use of next generation sequencing technology in plant breeding. | Reading of the papers about the lecture. | |
| 15 | General review | Reading of the papers about the lecture. | |
| 16 | Final Exam | Preparation to final exam | |
| 17 | Final Exam | Preparation to final exam |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 40 |
| General Assessment | ||
| Midterm / Year Total | 100 | 40 |
| 1. Final Exam | - | 60 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 78 | ||
| Total Workload / 25 (h) | 3,12 | ||
| ECTS | 3 ECTS | ||