TÜRKÇE
ENGLISH
Information
| Unit | FACULTY OF SCIENCE AND LETTERS |
| CHEMISTRY PR. | |
| Code | KM318 |
| Name | Biochemistry 2 Lab. |
| Term | 2019-2020 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Üniversite Dersi |
| Type | Normal |
| Label | C Compulsory |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. RAMAZAN BİLGİN |
| Course Instructor |
Prof. Dr. SEYDE SEYHAN TÜKEL
(Bahar)
(A Group)
(Ins. in Charge)
Prof. Dr. RAMAZAN BİLGİN (Bahar) (A Group) (Asst. Ins.) Prof. Dr. GÜZİDE YÜCEBİLGİÇ (Bahar) (A Group) (Asst. Ins.) |
Course Goal / Objective
Application of biochemical analysis methods, evaluation and interpretation of experimental results with theoretical knowledge.
Course Content
Determination of vitamin C in fruit juice, Determination of urea in serum, Isolation of DNA, Determination of Hemoglobin in whole blood, Polarimeter experiment, Determination of molecular weight of proteins with SDS-PAGE electrophoresis, Lowry protein method, Lowry protein method, Immobilization of lipase onto Celite.
Course Precondition
Yok
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learn biochemical analysis methods |
| LO02 | Evaluate and interpret experimental results |
| LO03 | Learn isolation of DNA |
| LO04 | Learn determination of molecular weight of proteins with SDS-PAGE electrophoresis |
| LO05 | Learn Lowry protein method |
| LO06 | Learn determination of lipase activity |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Determination of vitamin C in fruit juice | Biochemistry books, lecture notes | |
| 2 | Determination of urea in serum | Biochemistry books, lecture notes | |
| 3 | Isolation of DNA | Biochemistry books, lecture notes | |
| 4 | Determination of Hemoglobin in whole blood | Biochemistry books, lecture notes | |
| 5 | Polarimeter experiment | Biochemistry books, lecture notes | |
| 6 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis | Biochemistry books, lecture notes | |
| 7 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis | Biochemistry books, lecture notes | |
| 8 | Mid-Term Exam | Study related lecture notes | |
| 9 | Lowry protein method | Biochemistry books, lecture notes | |
| 10 | Determination of lipase activity by titration | Biochemistry books, lecture notes | |
| 11 | Immobilization of lipase onto Celite | Biochemistry books, lecture notes | |
| 12 | Immobilization of lipase onto Celite | Biochemistry books, lecture notes | |
| 13 | Immobilization of lipase onto Celite | Biochemistry books, lecture notes | |
| 14 | Make-up experiments | Biochemistry books, lecture notes | |
| 15 | Make-up experiments | Biochemistry books, lecture notes | |
| 16 | Term Exams | Study related lecture notes | |
| 17 | Term Exams | Study related lecture notes |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 20 |
| General Assessment | ||
| Midterm / Year Total | 100 | 20 |
| 1. Final Exam | - | 80 |
| Grand Total | - | 100 |