TÜRKÇE
ENGLISH
Information
| Unit | FACULTY OF AGRICULTURE |
| GARDEN PLANTS PR. | |
| Code | BBP352 |
| Name | Molecular Breeding Methods in Horticulture |
| Term | 2020-2021 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | E Elective |
| Mode of study | Uzaktan Öğretim |
| Catalog Information Coordinator | Prof. Dr. SALİH KAFKAS |
| Course Instructor |
Prof. Dr. YILDIZ KAÇAR
(Bahar)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
Defination and applications of biotechnology, the use of biotechnology in plant breeding
Course Content
This lesson contains the definition and application areas of biotechnology, tissue culture laboratory organization, embryo rescue and embryo culture, somatic embryogenesis and organogenesis, obtaining haploid plants, protoplast culture and somatic hybridization, plants, gene transfer methods, molecular genetics laboratory organization, DNA, RNA structures Investigation, PCR mechanism genetic markers: morphological and biochemical markers, molecular marker technology and GMOs, and the situation of the world.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Compare DNA and RNA in terms of similarities and differences. |
| LO02 | Defines molecular marker techniques. |
| LO03 | Defines and tests Polymerase Chain Reaction. |
| LO04 | Explains the methods of electrophoresis |
| LO05 | Memorises molecular breeding methods based on markers in horticultural plant breeding. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | To have sufficient theoretical knowledge in basic engineering and agricultural engineering | 1 |
| PLO02 | - | To have sufficient theoretical knowledge in basic horticulture and also fruit, vegetable, vitis and ornamental growing and breeding subjects | 5 |
| PLO03 | - | To have technical kknowledge in subjects related to horticulture | 4 |
| PLO04 | - | Finding the source of environmental, biological, technical and economical problems in horticulture those reduce yield and quality and developing solutions based on researches | 4 |
| PLO05 | - | To define, entitle and grow fruit, vegetable, vineyard and ornamentals | 4 |
| PLO06 | - | Breeding in Horticulture, developing new cultivars and producing propagation materials of new developed varieties | 4 |
| PLO07 | - | Using and applying biotechnology in horticulture | 5 |
| PLO08 | - | Applying methods for preserving genetic resources and environment for sustainable usage in horticulture | 5 |
| PLO09 | - | Irrigation, fertilization, defining pests and diseases, considering and assaying changes after post-harvest storage in horticulture | 1 |
| PLO10 | - | Obatining data in horticulture, researching, considering, recording, consulting and expert ability, project writing and applying | 3 |
| PLO11 | - | Recognizing different roles those enchance the individual skills and undertaking role as a member of the work team and having skills in terms of team work under different conditions | 3 |
| PLO12 | - | Accessing to the relevant information for the purpose, analytical thinking and planning, questioning, having an exploratory and creative vision and using the obtained knowledge for the benefit of society | 3 |
| PLO13 | - | Transfering knowledge to filed working people with different levels, concacting, data presenting. Moreover updating theirselves in terms of science, culture, art and social areas regarding their individual interest and skills. | 2 |
| PLO14 | - | Establishing-managing orchards, greenhouses and vineyards | 0 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA and RNA structure | Reading of the papers about the lecture. | |
| 2 | DNA and RNA isolation and methods used for determination the amount of DNA and RNA | Reading of the papers about the lecture. | |
| 3 | The genetic markers and the history | Reading of the papers about the lecture. | |
| 4 | PCR technologies and the use area | Reading of the papers about the lecture. | |
| 5 | Electrophoresis systems | Reading of the papers about the lecture. | |
| 6 | Molecular marker technologies and the use in the plant breeding | Reading of the papers about the lecture. | |
| 7 | PCR-based molecular markers | Reading of the papers about the lecture. | |
| 8 | Mid-Term Exam | Reading of the papers about the lecture. | |
| 9 | The creation of genetic maps | Reading of the papers about the lecture. | |
| 10 | Mid-Term Exam. | Preparation to mid-term exam. | |
| 11 | The use of genetic maps in plants breeding | Reading of the papers about the lecture. | |
| 12 | Marker assistant selection for plant breeding | Reading of the papers about the lecture. | |
| 13 | Molecular methods used in determination gene expression in plant and the use in plant breeding | Reading of the papers about the lecture. | |
| 14 | The use of next generation sequencing technology in plant breeding. | Reading of the papers about the lecture. | |
| 15 | General review | Reading of the papers about the lecture. | |
| 16 | Term Exams | Preparation to final exam | |
| 17 | Term Exams | Preparation to final exam |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 40 |
| General Assessment | ||
| Midterm / Year Total | 100 | 40 |
| 1. Final Exam | - | 60 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 78 | ||
| Total Workload / 25 (h) | 3,12 | ||
| ECTS | 3 ECTS | ||