BK570 Methods for the Diagnosis of Plant Pathogen Bacteria II

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code BK570
Name Methods for the Diagnosis of Plant Pathogen Bacteria II
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. YEŞİM AYSAN


Course Goal / Objective

the aims of the course are learning on identification of pathogenic bacteria, and confirmation of the identification by serological and molecular methods

Course Content

Identification of plant pathogenic bacteria and confirmation of the identification by serological (ELISA, IF, Immunostrip) and molecular methods

Course Precondition

none

Resources

Z. Klement, K. Rudolph,D.C. Sands, 1990. Methods in Phytobacteriology. Akademial Kiodo, Budapest. 567p. Lelliott, R. A., and D. E. Stead, 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Methods in Plant Pathology Volume 2 (Series editor T. F. Preece), Published on behalf of the British Society for Plant Pathology by Blackwell Scientific Publications, Oxford, UK., 219 pages. Saygılı, H., Şahin, F. and Aysan Y., 2006. Fitobakteriyoloji. Meta Basım Matbaacılık, İzmir, 530s.

Notes

Agrios, G.N. 2005. Plant Pathology. Elsevier Academic Press, London, England. pp: 639-647.


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Students will learn about bacterial isolation steps
LO02 Students will learn about identification of the bacteria by traditional methods
LO03 Students will learn about confrimatory tests of traditional identification tests
LO04 Students will learn about ELISA test in phytobacteriology
LO05 Students will learn about Immunofluorescent (IF) test in phytobacteriology
LO06 Students will learn about immunostrip test in phytobacteriology
LO07 Students will learn about bacterial identification by protein and lipopolysaccaride (LPS) anaylsis
LO08 Students will learn about bacterial identification by whole cell fatty acid analysis
LO09 Students will learn about usage of bacteriophages in phytobacteriology
LO10 Students will learn about usage of bioluminescent and flow cytometri in phytobacteriology
LO11 Students will learn about nucleic acid-based microbial identification methods
LO12 Students will learn about isolation of bacterial DNA
LO13 Students will learn about bacterial identification by polymerase chain reaction (PCR)
LO14 Students will learn about genotypic diversity between bacterial species by multi locus sequence analysis (MLST)
LO15 Students will learn about advanced integrated pathogen identification


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Has knowledge on basic topics about general science, general agricultural engineering and plant production 1
PLO02 Yetkinlikler - Öğrenme Yetkinliği Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants 2
PLO03 Yetkinlikler - Öğrenme Yetkinliği Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice
PLO04 Yetkinlikler - Öğrenme Yetkinliği Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods 2
PLO05 Beceriler - Bilişsel, Uygulamalı Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports 3
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly 3
PLO07 Yetkinlikler - Öğrenme Yetkinliği Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations 3
PLO08 Bilgi - Kuramsal, Olgusal By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education
PLO09 Yetkinlikler - Alana Özgü Yetkinlik He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in.
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Can present results of the scientific research as a report/thesis and produce scientific publications
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production.
PLO12 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems.
PLO13 Yetkinlikler - Öğrenme Yetkinliği Develops skills in using laboratory methods and techniques
PLO14 Yetkinlikler - Alana Özgü Yetkinlik Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection.


Week Plan

Week Topic Preparation Methods
1 Bacterial isolation steps Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
2 Identification of the bacteria by traditional methods Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
3 Confrimatory tests of traditional identification tests Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
4 ELISA test in phytobacteriology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
5 Immunofluorescent (IF) test in phytobacteriology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
6 Immunostrip test in phytobacteriology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
7 Bacterial identification by protein and lipopolysaccaride (LPS) anaylsis Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam preparing the exam Ölçme Yöntemleri:
Sözlü Sınav
9 Bacterial identification by whole cell fatty acid analysis Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
10 Usage of bacteriophages in phytobacteriology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
11 Usage of bioluminescent and flow cytometri in phytobacteriology Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
12 Nucleic acid-based microbial identification methods Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
13 Isolation of bacterial DNA (genomic-DNA) Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
14 Bacterial identification by polymerase chain reaction (PCR) Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
15 genotypic diversity between bacterial species by multi locus sequence analysis (MLST) Reading the related lecture notes Öğretim Yöntemleri:
Anlatım
16 Term Exams preparing the exam Ölçme Yöntemleri:
Sözlü Sınav
17 Term Exams preparing the exam Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 16.11.2022 10:49