TÜRKÇE
ENGLISH
Information
| Code | BK049 |
| Name | Application of Biotechnological Methods on Fungi |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUKADDES KAYIM |
Course Goal / Objective
The aim of this course is to teach the biotechnological methods in plant pathogen fungi. For this purpose, it is to amplify the spesific genes used in molecular diagnostic by PCR-based methods and to perform phylogenetic tree by nucleotid alignment and to compare the results with molecular and traditional methods (morphological, cultural and physiological)
Course Content
Introduction and classification of fungi, molecular characterization of fungi purified from diseased plant tissue, phylogenetic analysis, classification by gene groups, gene transfer in fungi and application areas and methods of biotechnological methods
Course Precondition
Plant Fungi Diseases requirement
Resources
Introduction to Fungi (John Webster and Roland Weber, 2007 Cambridge University Press), Modern Mycology (J.W. Deacon, 2001, 3. Edition)Modern Mycology (J.W. Deacon, 2001, 3. Edition))
Notes
Courses notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns microscopy methods in fungi. Learns pathogen isolation and purification from diseased plants and soil. |
| LO02 | Learns nucleic acid isolation methods from fungal cultures.Learns the characteristics of genes involved in fungal diagnosis. |
| LO03 | Learns how to amplify important genes for diagnosis by PCR-based methods.Learns the comparison and matching analysis of the replicated genes in the gene database |
| LO04 | Learns to compare classical and molecular methods in the diagnosis of plant pathogen fungi |
| LO05 | Learn how to use genetically modified transgenic plants to control plant pathogenic fungi |
| LO06 | Understands the importance of molecular methods in the classification of fungi.Learns the cultural and physical characteristics of fungi. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Has knowledge on basic topics about general science, general agricultural engineering and plant production | 1 |
| PLO02 | Yetkinlikler - Öğrenme Yetkinliği | Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants | 3 |
| PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice | |
| PLO04 | Yetkinlikler - Öğrenme Yetkinliği | Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can present results of the scientific research as a report/thesis and produce scientific publications | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develops skills in using laboratory methods and techniques | |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General characteristics of plant pathogen fungi | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Classical classification of plant pathogenic fungi | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 3 | Overview of molecular methods used in the classification of plant pathogenic fungi | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri |
| 4 | The roles of Internal transcribed spacer (ITS), Elongation factor 1, Beta tubulin (TUB2), SSU, LSU, Actin (ACT), Calmodulin (CAL), Chitin syntase (CHS-1), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glutamine synthase (GS) genes in the classification of plant pathogen fungi | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri, Gösterip Yaptırma |
| 5 | Isolation and purification methods of plant pathogenic fungi from diseased plant tissues | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 6 | Determination of cultural and physical characteristics of purified fungi | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 7 | DNA isolation methods from pure fungus cultures and PCR techniques for DNA amplification | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri, Gösterip Yaptırma |
| 8 | Mid-Term Exam | Written exam | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 9 | Methods for amplificaion of Internal transcribed spacer (ITS), Elongation factor 1, Beta tubulin (TUB2), SSU, LSU, Actin (ACT), Calmodulin (CAL), Chitin syntase (CHS-1), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glutamine synthase (GS) genes by PCR | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 10 | Comparison analysis of gene sequences in fungal diagnosis and classification | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 11 | Fungal identification and classification of cultural and morphological methods combined with molecular methods | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 12 | Filogentic analysis of genes-1 (According to Fungus sections) | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 13 | Classification according to phylogenetic analysis (examples) | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 14 | Phylogenetic tree methods for fungal classification | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 15 | Development of fungi-resistant plants by molecular and biotechnological methods, GMO | Course notes and related books and articles | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 16 | Term Exams | Written exam | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 17 | Term Exams | Written exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||