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Information
| Code | BY653 |
| Name | Micropreparation Tecniques in Animal Tissues |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Dr. Öğr. Üyesi SAADET PINAR ÖZALP |
Course Goal / Objective
The aim of this course is to teach the students micropreparation methods
Course Content
Preparate tecniques of animal tissue, total preparation methods, dry off, frot method, fixation, commonly used fixative mixtures, dehydration, embedding with paraffin, sectioning, staining, mounting after staining.
Course Precondition
None
Resources
Lecture notes
Notes
Micropreparation methods ( Prof.Dr.Neriman Ozban)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Understand the preparate tecniques of animal tissue. Comprehends preparation techniques and applications. Comprehends the total preparation method in animal tissues. |
| LO02 | Understands the dry closing method in animal tissues. Understand the frot method. Understand the fixation, Understand the commonly used fixative mixtures. |
| LO03 | Understand the preparation of Understand the staining of sections sections, Understand the closure of preparations after staining. |
| LO04 | Learn microscopes and special microscopy methods used for biological purposes. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Identifies the interdisciplinary interaction that the field is related to. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Interprets the knowledge gained in the field by integrating the information from different disciplines and creates new information. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Carries out a study that requires expertise related to the field independently. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Evaluates the knowledge and skills acquired at the level of expertise in the field with a critical approach and directs its learning. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Supports the current developments in the field and their studies with quantitative and qualitative data and transmits them both in the field and outside the field systematically in written, oral and visual form. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | It supervises the social, scientific, cultural and ethical values in the collection, interpretation, application and announcement stages of the data related to the field and teaches these values. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | The information they absorbed in the field; use problem solving and / or practical skills in interdisciplinary studies. | 3 |
| PLO09 | Belirsiz | It contributes to the solution of social, scientific, cultural and ethical problems encountered in the field and supports the development of these values. | |
| PLO10 | Belirsiz | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Comprehend the issues that need to be prepared and paid attention to the working laboratory. | Source reading | Öğretim Yöntemleri: Anlatım |
| 2 | Understands the preparation of stock melts, buffer melts and dye solutions. | Source reading | Öğretim Yöntemleri: Anlatım |
| 3 | Comprehend dry closing, Frotis method, crushing preparation method. | Source reading | Öğretim Yöntemleri: Anlatım |
| 4 | Comprehend the fixation method. | Source reading | Öğretim Yöntemleri: Anlatım |
| 5 | Comprehends the widely used fixative mixtures and their preparation. | Source reading | Öğretim Yöntemleri: Anlatım |
| 6 | Comprehends washing and dehydration of tissues. | Source reading | Öğretim Yöntemleri: Anlatım |
| 7 | Understands the method of transparency and the solutions used. | Source reading | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Source reading | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Understands the preparation and sectioning of paraffin sections. | Source reading | Öğretim Yöntemleri: Anlatım |
| 10 | Understands the cleaning of microtomes, microtome blades, and slides | Source reading | Öğretim Yöntemleri: Anlatım |
| 11 | Comprehend the paints and the mechanism of painting. | Source reading | Öğretim Yöntemleri: Anlatım |
| 12 | Comprehend the methods of metachromasia, mordanting. | Source reading | Öğretim Yöntemleri: Anlatım |
| 13 | Comprehend the closure and labeling methods of preparations. | Source reading | Öğretim Yöntemleri: Anlatım |
| 14 | Comprehend vital and supravital examination, tissue culture methods. | Source reading | Öğretim Yöntemleri: Anlatım |
| 15 | Comprehend the chromatography method. | Source reading | Öğretim Yöntemleri: Anlatım |
| 16 | Comprehend the electrophoresis method. | Source reading | Öğretim Yöntemleri: Anlatım |
| 17 | Term Exams | Source reading | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||