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Information
| Code | BT0137 |
| Name | Cell Culture and Somatic Hybridization Applications in Plant Breeding |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
This course covers cell culture techniques used in plant breeding, protoplast isolation in plants, and protoplast hybridization in plants, as well as determining the polydi levels of individuals obtained by cell culture in plant breeding.
Course Content
Cell culture in plants, media used in plant cell culture and its preparation, selection and creation of appropriate plant tissue, somatic embryogenesis, ovule culture, plant tissue selection, protoplast extraction, protoplast isolation, protoplast purification, protoplast culture, somatic hybridization, protoplast culture media, somatic hybridization methods, protoplast culture, heterokaryon culture, micro callus culture, plant regeneration from cell, flow cytometry
Course Precondition
None
Resources
Lecture notes, books, research articles, periodicals on the subject
Notes
Internet resources obtained from scientific search engines
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the use of cell culture in plant breeding |
| LO02 | Knows cell culture techniques in plants |
| LO03 | Develops the ability to interpret, evaluate and construct constructive criticisms of scientific writings |
| LO04 | Knows the methods of using flow cytometry in plant breeding |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | 2 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | 3 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | 1 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | 1 |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Plant Cell Culture | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Gösteri, Anlatım, Tartışma |
| 2 | Usage of plant cell culture in plant breeding | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 3 | Protoplast as a concept | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Gösteri, Anlatım, Tartışma |
| 4 | Protoplast culture methods | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 5 | Plant protoplast sources | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 6 | Obtaining protoplasts from plants | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 7 | Somatic hybridization in plants | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-term exam | Preparation to mid-term exam | Ölçme Yöntemleri: Ödev |
| 9 | Purification and counting of protoplasts | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Cell loads and cell counts suitable for fusion | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 11 | Protoplast fusion methods | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 12 | Regenerations of heterokaryons | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Cultivation techniques, media used and regeneration of heterokaryons | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Flow cytometry in plant breeding | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri, Gösterip Yaptırma |
| 15 | Identification of plants from heterokaryons by flow cytometry | Reading recommended books and given lecture notes | Öğretim Yöntemleri: Tartışma, Anlatım, Gösteri |
| 16 | Final examination | Preparation to final exam | Ölçme Yöntemleri: Ödev |
| 17 | Final examination | Preparation to final exam | Ölçme Yöntemleri: Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 152 | ||
| Total Workload / 25 (h) | 6,08 | ||
| ECTS | 6 ECTS | ||