TÜRKÇE
ENGLISH
Information
| Code | BT0099 |
| Name | Animal Cell Culture Applications-I |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
Learning the Application Areas of Basic Methods in Cell Culture
Course Content
Cell culture Techniques, Devices Used in Cell Culture and Their Properties, Cell Culture Sowing, Propagation, Passaging and Freezing Methods, Cell Culture Safety Conditions
Course Precondition
none
Resources
Freshney RI., Culture of Animas Cells:A Manual of Basic Technique
Notes
Freshney RI., Culture of Animas Cells:A Manual of Basic Technique
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Can choose a medium to support cell culture |
| LO02 | Has knowledge of the basic features of cell culture |
| LO03 | Knows the differences of cell culture types and has information about comparing cell structures |
| LO04 | Has the knowledge to choose the appropriate medium for the structure of the cells according to their types |
| LO05 | Define the coordination of medium and support medium in cell culture. |
| LO06 | Can identify contaminations that may occur in cell culture |
| LO07 | Determine what contamination may be in cell culture and evaluate the appropriate solution. |
| LO08 | Has knowledge of the safety of the person working in cell culture applications |
| LO09 | Define the methods that can be used in living cell determination |
| LO10 | Has knowledge about the methods of using cells obtained from cell culture in molecular studies. |
| LO11 | Has knowledge about the cryopreservation and storage conditions of sub-passages in cell culture |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | 5 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | 4 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. | 4 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | What is cell culture? What are the cell culture uses? | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Cell culture types | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Techniques used in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Cell culture media | Literature browsing and reading | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 5 | Support media in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 6 | Cell culture contaminations | Literature browsing and reading | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 7 | Measures to be taken against cell culture contamination | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | midterm | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 9 | Safety conditions in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Cell viability determination methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Molecular methods that can be used after Cell Culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Cell culture freezing methods | Literature browsing andreading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Storage conditions of frozen cells | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Cell count | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Flask selection according to application method in cell culture | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 17 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||