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Information
| Code | BT0096 |
| Name | Polymerase Chain Reaction (PCR), display of the Optimization Results |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
The aim of the course is to enable the graduates to have knowledge about Polymerase Chain Reaction, to be able to apply the test and to solve the problems they may encounter during the applications.
Course Content
The components of this course consist of Polymerase Chain Reaction, it's standardisation and the evaluation of the test results.
Course Precondition
NONE
Resources
Lecture notes and presentations will be used on for the courses
Notes
Freeman S, Herron JC, Evrimsel Analiz, Palme Yayıncılık, Ankara, 2009. Lemey P, Salemi M, Vandamme AM, The Phylogenetic Handbook, A Practical Approach to Phylogenetic Analysis and Hypothesis Testing, Cambridge University Press, 2009. Pevsner J, Bioinformatics and Functional Genomics, Wiley-Blackwell, 2009. Xıong J, Essential Bioinformatics, Cambridge University Press, 2006.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Student can perform electrophoresis and imaging processing of PCR products |
| LO02 | Students will solve the problems while they are using PCR and optimise the test |
| LO03 | Students will have a knowledge about PCR and PCR based molecular techniques |
| LO04 | Students will learn DNA ekstraction from the tissues |
| LO05 | Students will learn the molecular structure of the cell |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 3 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 3 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 3 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 2 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 2 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Structure of the cell | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | The structure and replication of of DNA | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Transcription and translation | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Gen, Genom and Regulation of DNA | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Mutations (Genom, Gen and DNA mutations) | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | DNA extraction methods | DNA extraction methods | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Introduction to Polymerase Chain Reaction (PCR) | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Mid-Term Exam | Ölçme Yöntemleri: Sözlü Sınav, Ödev |
| 9 | Modifications of PCR (Semi-Nested PCR, Nested PCR, Multiplex-PCR, RAPDPCR, Real Time-PCR) | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Optimisation in PCR ( enzyme, buffer, magnesium chloride, potassium chloride, dNTP mix, primers) | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Optimisation in PCR (Optimisation of PCR cycles) | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Preparation of gels (agarose, PAGE) that used in the electrophoresis of the amplicons | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Electrophoresis, staining of PCR gels and screanin of the results | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Optimisation in the electrophoresis, jel preparation and screening | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Problems in PCR and solution offers | Lecture notes,slide projection | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Term Exams | Ölçme Yöntemleri: Sözlü Sınav, Ödev |
| 17 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||