BT077 Immunohistochemical and Immunocytochemical Techniques in Animal Tissues

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code BT077
Name Immunohistochemical and Immunocytochemical Techniques in Animal Tissues
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. MEHMET HALIGÜR


Course Goal / Objective

The aim of this course is to determine the reactions which can be determined by immunohistochemical method in animal tissues or animal cells.

Course Content

In this course, antigen-antibody complexes in animal tissues, immunohistochemical or immunocytochemical detection of infectious agents and tumor markers, immunohistochemistry and immunocytochemistry method, types and applications and immunohistochemical characteristics will be given.

Course Precondition

None

Resources

Lin F, Prichard J. 2015. Handbook of Practical Immunohistochemistry, Springer; Dabbs D. 2021. Diagnostic Immunohistochemistry, 6th Edition, Elsevier.

Notes

Lecture notes and current literature


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Students will learn to apply immunohistochemical methods in animal tissues
LO02 Learns what can be done in tissues by immunohistochemical method
LO03 In experimental studies, how to determine the infectious diseases and determine the molecular changes in tissues
LO04 Learns determination of proteins or receptors in tissues
LO05 Identify antigen-antibody complexes and make them visible or detectable by light microscope
LO06 Learn the types and stages of immunohistochemical and immunocytochemical methods


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. 3
PLO02 Beceriler - Bilişsel, Uygulamalı To identifiy the interdisciplinary interaction of the field and to solve use of the methods. 5
PLO03 Beceriler - Bilişsel, Uygulamalı Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information 4
PLO04 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. 2
PLO05 Yetkinlikler - Öğrenme Yetkinliği To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. 3
PLO06 Yetkinlikler - İletişim ve Sosyal Yetkinlik To solve the problems encountered in the field and cominicate by using research methods. 2
PLO07 Yetkinlikler - Alana Özgü Yetkinlik To gain the ability to develop and deepen the knowledge in the field of biotechnology 5
PLO08 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Knows the ethical rules to be considered while obtaining biotechnological products.
PLO10 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.


Week Plan

Week Topic Preparation Methods
1 Definition of immunohistochemistry, general feature, what is included in the content Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
2 Definition of immunohistochemistry and the properties and types of hyperimmune sera Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
3 The application areas of immunohistochemistry, how to apply to tissues, how to apply to cytological preparations Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
4 The application areas of immunohistochemistry, what are the considerations when touching, what are the considerations when applying to cytological preparations. Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
5 Types of immunohistochemistry and immunocytochemistry, PAP and APPAP methods, how to apply Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
6 immunohistochemically identification of infectious agents, in particular bacteria such as actinomyces pyognes, which cause pathological effects in animal tissues, identification of intercellular bacteria such as mycobacterium Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
7 immunohistochemically determining cell receptors; The role of immunohistochemistry in the detection and detection of receptors such as TNF-alpha, Fas-Ligand Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
8 Mid-Term Exam Mid-Term Exam Ölçme Yöntemleri:
Yazılı Sınav, Sözlü Sınav
9 Determination of cell death pathways by immunohistochemical method, mechanism of action of caspases, effect of caspases on cell death Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
10 Use of tumor cell markers as immunohistochemically; PCNA refers to Ki-67, which markers are used in epithelial tumors Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
11 The use of tumor cell markers in immunocytochemistry, which markers are used in mesenchymal origin tumors, which markers are used in smears, Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
12 The markers used in the application of immunohistochemical methods, markers used in gland tumors, the markers of hemotopeoietic system cells Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
13 The markers used in the application of immunohistochemical method; What are the ingredients used in bone tissue and tu Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
14 Immunohistochemistry and immunocytochemistry application; application stages, what are the points to be considered at these stages and the solution of the problems related to these stages, Lecture notes Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma, Problem Çözme
15 Immunohistochemistry and immunocytochemistry application; ways to solve problems, evaluation of preparations and how to prevent incorrect evaluations Lecture notes Öğretim Yöntemleri:
Anlatım, Problem Çözme
16 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 14.11.2022 10:46