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Information
| Code | BFZ513 |
| Name | Flourescence imaging techniques in the cell |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. FİGEN ÇİÇEK |
Course Goal / Objective
Learning the fluorescence imaging techniques in the cell and perform fluorescence imaging in the cell.
Course Content
Fluorescence and its physics principles. Chemical fluorescence probes and their cellular usages. Measurement of the basic ions with the fluorescent techniques in the cells Dynamic measurement of the cellular signalling pathways with fluorescent probes. Biological fluorescent probes and their cellular usages. Other techniques; FRET, Fluorescent life time microscopes and immunofluorescent techniques.
Course Precondition
The lesson has no prerequisites
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns fluorescence with its physics principles. |
| LO02 | Learns different fluorescence imaging techniques. |
| LO03 | Learns to decide florophore, type of the florescent system, wavelength and filters according to the experimental system. |
| LO04 | Learns image data analysis. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns basic information about the field | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Sets up and works experimental setups in the laboratory | 5 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Prepares a project proposal on its own | 5 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Plans an experimental study | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Reports the research result | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Interpret the research findings | 5 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Has the analytical thinking mechanism related to the field, Reaches reliable information. Creates and writes a research project, works and researches based on ethical values. | 5 |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | She/he systematically transfers the theoretical knowledge she has researched to the audience. | 4 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Have a scientific thinking systematic in relation to their profession. | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Basics of the Fluorescence | Reads the related chapter from the textbook and lecture notes. | |
| 2 | Biological fluorescence probes and their cellular usages | Reads the related chapter from the textbook and lecture notes. | |
| 3 | Chemical fluorescent dyes and their types and usages | Reads the related chapter from the textbook and lecture notes. | |
| 4 | Fluorescent measurement systems and their working principles 1 Fluorimeters | Reads the related chapter from the textbook and lecture notes. | |
| 5 | Fluorescent measurement systems and their working principles 2 Camera and confocal systems | Reads the related chapter from the textbook and lecture notes. | |
| 6 | Ion imaging in the cell. | Reads the related chapter from the textbook and lecture notes. | |
| 7 | Calibration in the fluorescent measurements | Reads the related chapter from the textbook and lecture notes. | |
| 8 | Mid-Term Exam | ||
| 9 | Fluorescent imaging and Data analysis study | Reads the related chapter from the textbook and lecture notes | |
| 10 | Measurement of dynamic ion concentrations in the celli | Reads the related chapter from the textbook and lecture notes | |
| 11 | LASER as a light source | Reads the related chapter from the textbook and lecture notes | |
| 12 | Fluorescent meaurement of DNA and immunofluorescent techniques | Reads the related chapter from the textbook and lecture notes | |
| 13 | FRET and Fluoresence lifetime imaging microscopy | Reads the related chapter from the textbook and lecture notes | |
| 14 | Flow cytometry | Reads the related chapter from the textbook and lecture notes | |
| 15 | A fluorescence system design project work | Reads the related chapter from the textbook and lecture notes | |
| 16 | Term Exams | ||
| 17 | Term Exams |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 118 | ||
| Total Workload / 25 (h) | 4,72 | ||
| ECTS | 5 ECTS | ||