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Information
| Code | HİS628 |
| Name | Application of Molecular Biology Techniques |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. LEMAN SENCAR GÜNDOĞDU |
| Course Instructor |
1 |
Course Goal / Objective
Getting ability to applicate the techniques that are used in molecular biology
Course Content
Equipments that are used in molecular biology laboratory, DNA isolation, agarose gel electrophoresis, application of PCR, isolation and analysis of RNA, application of blotting and ELISA.
Course Precondition
None
Resources
Michael H. Ross Histoloji Lecture and Atlas
Notes
Lecture notes and slides
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knowing and using equipment of molecular biology laboratory. |
| LO02 | Preparing buffer solutions. |
| LO03 | Application of DNA isolation and agarose gel electrophoresis. |
| LO04 | Application of polimerase chain reaction. |
| LO05 | Application of RNA isolation and analysis |
| LO06 | Application of ELISA method. |
| LO07 | Application of blotting methods. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | 1 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | 4 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Demonstration of molecular biology laboratory equipments | Self-study, reading recommended references | Öğretim Yöntemleri: Gösteri |
| 2 | Application of DNA isolation | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 3 | Application of agarose gel electrophoresis technique | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 4 | PCR opmitimisation, application of gradient PCR | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 5 | Cutting of PCR products with using restriction enzymes, displaying these products by polyacrilamide gel electrophoresis | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 6 | Reuptaking PCR products from the gel,purification process, analysing in agarose gel | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 7 | Isolation and analysis of RNA | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Converting the RNA's to cDNA and spectral analysis | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Designing specific primers for different genomic regions by using cDNA as a template and application of PCR | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 11 | Denaturing gel electrophoresis | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 12 | Application of blotting techniques; Western Blotting, Southern Blotting | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 13 | Methods for protein isolation and quantification | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 14 | Measuring protein concentrations in serum by ELISA | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 15 | Seminar | Self-study, reading recommended references | Öğretim Yöntemleri: Gösteri |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 108 | ||
| Total Workload / 25 (h) | 4,32 | ||
| ECTS | 4 ECTS | ||