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Information
| Code | HİS624 |
| Name | Practice of The Histology Laboratory Techniques |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. YURDUN KUYUCU |
| Course Instructor |
1 |
Course Goal / Objective
Aim of this course is to apply tissue preparation and examination techniques.
Course Content
Light and electron microscopic tissue preparation, sectioning and staining, applying immunohistochemical and histochemical techniques..
Course Precondition
Histology Laboratory Techniques
Resources
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Notes
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows how to use a light microscope. |
| LO02 | Prepares tissue fixation solutions for light and electron microscopy. |
| LO03 | Applies fixation techniques. |
| LO04 | Applies dehydration and clearing tissue sections. |
| LO05 | Be able to embed the tissue sample appropriately. |
| LO06 | Sectioning by microtome and ultramicrotome. |
| LO07 | Applies staining methods. |
| LO08 | Applies histochemical staining methods. |
| LO09 | Takes photos of the specimens on light microscope. |
| LO10 | Offers solutions about problems of the technical steps. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | 5 |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Preparation of the solutions used in light and electron microscopic tissue processing | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 2 | Obtaining tissues for light and electron microscopic examination | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 3 | The application of light microscopic tissue processing steps | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 4 | Sectioning with microtome | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 5 | Staining of the sections with H.E. | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 6 | The application of the connective tissue stains | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 7 | The application of the nerve tissue stains | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Application of staining methods for nuclear and cytoplasmic components | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Application of staining methods for enzymes, carbohydrates and lipids | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 11 | Evaluation of the slides that examined and photographed under a microscope | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 12 | Application of the electron microscopic tissue processing | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 13 | Sectioning thin and semi-thin sections with ultramicrotome and staining | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 14 | Electron microscopic examination, photography | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 15 | Evaluation of electron microscopic photographes | Self-study | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 16 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 6 | 6 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 9 | 9 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||