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Information
| Code | HİS521 |
| Name | Histological Techniques I (Z) |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. YURDUN KUYUCU |
| Course Instructor |
1 |
Course Goal / Objective
Aim of this course is learning and applying tissue preparation techniques for light microscopic and electron microscopic evaluation of organs and tissues .
Course Content
Microscope types, light and electron microscopic tissue preparation and staining protocols and histochemical techniques
Course Precondition
None
Resources
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Notes
Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows how to use a light microscobe. |
| LO02 | Prepares solutions for light and electron microscopic tissue preperation. |
| LO03 | Applies fixation techniques |
| LO04 | Applies dehydration and clearing of a tissue section. |
| LO05 | Be able to embed the tissue sample appropriately. |
| LO06 | Applies sectioning from a microtome and ultramicrotome. |
| LO07 | Applies staining methods. |
| LO08 | Knows the basics of histochemical staining methods. |
| LO09 | Knows the histochemical staining methods for nucleus and cytoplasmic organelles, connective tissue and enzymes. |
| LO10 | Takes photos of the specimen on light microscope. |
| LO11 | Offers solutions to problems at any technique step of the staining and preparation method. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Tell the basic histological features of cells and tissues. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Can establish connection betwwen the structure and function of the cells. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Have knowledge about the histological properties of organ systems. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Have knowledge about embryo development, development disorders and mechanisms. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Define light and electron microscopic tissue preparation and examination methods. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has knowledge about general principles of cell culture, histochemistry and immunohistochemistry. | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Prepare tissues for light and electron microscopic analysis. | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Apply basic histochemical staining methods. | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Can distinguish tissue and organ systems at light microscopic level. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | He / she carries out his / her study as an individual or a team member. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Do the tasks given within the scope of research projects. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Develop a positive attitude and behavior related to lifelong learning. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Identifies learning needs and resources, and guides them by using resources effectively. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Evaluate the knowledge and skills at the graduate level with a critical approach. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Use information and communication technologies at the level required by the field. | |
| PLO16 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Communicate effectively. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transfer their ideas and solutions by quantitative and qualitative datas by supporting the current developments and their studies orally and visiually | |
| PLO18 | Yetkinlikler - Öğrenme Yetkinliği | Knows the importance of ethical principles in conducting research studies, evaluating and sharing the results. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General information about the methods of histological examination | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 2 | Microscope types and usage areas | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 3 | Light microscopic tissue preparation methods | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 4 | Light microscopic tissue staining methods | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 5 | Preparation methods for evaluation of hard tissues | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 6 | Special stains for determining the hematopoietic and lenforeticular tissue elements | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 7 | Basic principles of the histochemistry | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Histochemical techniques used for connective tissue | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 10 | Histochemical techniques used for nerve tissue | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 11 | Histochemical techniques used in demonstration of nuclear and cytoplasmic elements | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 12 | Histochemical techniques used for enzymes, carbohydrates and lipids | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 13 | Electron microscopic tissue preparation techniques | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 14 | Solution and stains used in electron microscopic tissue preparation | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 15 | Artefacts | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 20 | 20 |
| Final Exam | 1 | 21 | 21 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||