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Information
| Code | HİS513 |
| Name | Histological Techniques 2 |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. ÖZGÜL TAP |
Course Goal / Objective
Teaching the basic principles of immunohistochemistry and basic methods of molecular biology.
Course Content
İmmunohistokimyasal yöntemlerin temel prensipleri, kullanılan antikor tipleri, doku takibi, boyama metodları, değerlendirme kriterleri, moleküler biyolojide kullanılan temel yöntemler.
Course Precondition
none
Resources
Histoloji Konu Anlatımı ve Atlas-Ross
Notes
ders sunumları
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Defines immunohistochemistry |
| LO02 | Knows basic principles of immunohistochemical techniques |
| LO03 | Knows fixation and immunohistochemical labeling process |
| LO04 | Proposes solutions to problems faced while applying the technical steps |
| LO05 | Applies and comprehends all basic steps in immunohistochemistry techniques |
| LO06 | Knows basic methods and principles uses in molecular biology |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Tell the basic histological features of cells and tissues. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Can establish connection betwwen the structure and function of the cells. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Have knowledge about the histological properties of organ systems. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Have knowledge about embryo development, development disorders and mechanisms. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Define light and electron microscopic tissue preparation and examination methods. | 4 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has knowledge about general principles of cell culture, histochemistry and immunohistochemistry. | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Prepare tissues for light and electron microscopic analysis. | 4 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Apply basic histochemical staining methods. | 3 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Can distinguish tissue and organ systems at light microscopic level. | 2 |
| PLO10 | Bilgi - Kuramsal, Olgusal | He / she carries out his / her study as an individual or a team member. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Do the tasks given within the scope of research projects. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Develop a positive attitude and behavior related to lifelong learning. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Identifies learning needs and resources, and guides them by using resources effectively. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Evaluate the knowledge and skills at the graduate level with a critical approach. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Use information and communication technologies at the level required by the field. | |
| PLO16 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Communicate effectively. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transfer their ideas and solutions by quantitative and qualitative datas by supporting the current developments and their studies orally and visiually | |
| PLO18 | Yetkinlikler - Öğrenme Yetkinliği | Knows the importance of ethical principles in conducting research studies, evaluating and sharing the results. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The basic principles of immunohistochemistry | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 2 | Immunohistochemical labeling process | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 3 | The basic principles of tissue processing | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 4 | Monoclonal and polyclonal antibodies | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 5 | Determination of appropriate seconder antibodies | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 6 | Preparation of solutions used in the method | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 7 | Immunohistochemical staining procedure | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 9 | Evaluation criteria of slides in microscope | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 10 | Methods used in molecular biology -1 | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 11 | Methods used in molecular biology -2 | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 12 | Basic principles and application of polymerase chain reaction | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 13 | Western Blot technique | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 14 | Hybridization techniques | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 15 | ELISA technique | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Öğretim Yöntemleri: Anlatım |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 114 | ||
| Total Workload / 25 (h) | 4,56 | ||
| ECTS | 5 ECTS | ||