TÜRKÇE
ENGLISH
Information
| Code | BİO525 |
| Name | Cytogenetics (Z) |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-3 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYFER PAZARBAŞI |
| Course Instructor |
1 |
Course Goal / Objective
Demonstrating and presenting characteristics, mutations and cytogenetic analysis of chromosomes and pedigree analysis.
Course Content
Packaging of DNA and chromosome, characteristics, mutations and cytogenetic analysis of chromosomes, Pedigree analysis, Otosomal and sexual abnormalities, Genetic control of gender, Separation of chromosomes, Giant chromosomes, Barr body, Mendelian and non mendelian inheritance, Prenatal diagnosis and Genetic screening.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The student tells packaging of DNA and chromosome |
| LO02 | The student lists characteristics of chromosomes |
| LO03 | The student lists mutations of chromosomes |
| LO04 | The student distinguishes Cytogenetic analysis of chromosomes: lymphocyte culture and preparing metaphase plaque |
| LO05 | The student tells and applies karyotype analyses |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To comprehend the structure and functions of cells and organelles which constitute the basic structure of life | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To recognize the functions of tissue and organ systems | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To examine the structure of genetic material | 5 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To recognize genetic diseases and genes that cause these diseases | |
| PLO05 | Bilgi - Kuramsal, Olgusal | To learn the structure of genes | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To define chromosome and gene mutations | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To classify heredity forms (autosomal dominant, autosomal recessive, gender-dependent dominant and recessive inheritance models) | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To use molecular and cytogenetic analysis | |
| PLO09 | Bilgi - Kuramsal, Olgusal | To be able to explain the numerical and structural anomalies of autosomal and sex chromosomes | |
| PLO10 | Bilgi - Kuramsal, Olgusal | To make DNA isolation from all kinds of tissues | |
| PLO11 | Bilgi - Kuramsal, Olgusal | To prepare PCR | |
| PLO12 | Bilgi - Kuramsal, Olgusal | To make and interpret agarose and polyacrylamide gel electrophoresis | |
| PLO13 | Bilgi - Kuramsal, Olgusal | To measure the obtained DNA on the spectrophotometer | |
| PLO14 | Bilgi - Kuramsal, Olgusal | To apply and interpret QF-PCR technique | |
| PLO15 | Bilgi - Kuramsal, Olgusal | To make cell culture from blood, amnion, chorionic villus biopsy specimens and to obtain chromosome preparation | |
| PLO16 | Bilgi - Kuramsal, Olgusal | To perform karyotype analysis of chromosome preparations and to recognize and interpret all human chromosomes | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | To interpret a scientific article and to learn how to write a paper |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Packaging of DNA and chromosome | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 2 | Characteristics of chromosomes | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 3 | Mutations of chromosomes | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 4 | Mutations of chromosomes | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 5 | Cytogenetic analysis of chromosomes: lymphocyte culture and preparing metaphase plaque | Literature review and reading | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri |
| 6 | Analyses of karyotype and pedigree | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 7 | Otosomal abnormalities | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparation an presentation | Öğretim Yöntemleri: Anlatım |
| 9 | Sexual abnormalities and genetic control of gender | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 10 | Separation of chromosomes | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 11 | Giant chromosomes, Barr body | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 12 | Mendelian inheritance | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 13 | Non mendelian inheritance | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 14 | Prenatal diagnosis | Literature review and reading | Öğretim Yöntemleri: Tartışma |
| 15 | Genetic screening | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 8 | 8 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 123 | ||
| Total Workload / 25 (h) | 4,92 | ||
| ECTS | 5 ECTS | ||