BİO644 Tissue Culture

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit
Information About Course

Information
Goal, Content & Resources
Course Learning Outcomes
Relation with Program Learning Outcome
Week Plan
Student Workload - ECTS

Information

Code BİO644
Name Tissue Culture
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Doktora Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. AYFER PAZARBAŞI


Course Goal / Objective

To present and explain the tissue culture with every aspects.

Course Content

Learning and explaining the tissue culture with every aspects. Determine and design the requirements of tissue culture laboratory, beeing able to list and apply all needed techniques and conditions, beeing able to produce the cells and keep them in the term of cryopreservation.

Course Precondition

There is no precondition for this lesson.

Resources

Culture of animal cells: A Manual of Basic Technique, Fifth Edition, by R. Ian Freshney. Copyright 2005, John Wiley&Sons, Inc.

Notes

Methods in Molecular Biology, vol.290: Basic Cell Culture Protocols, Third Edition, Edited by CD Helgason and CL Miller. Humana Press, Inc., Totowa, NJ.


Course Learning Outcomes

Order Course Learning Outcomes
LO01 The student identifies the terminology of tissue culture.
LO02 The student sorts the criteria for tissue culture.
LO03 The student comprehends the effects and results of aseptic conditions and sterilization on tissue culture.
LO04 The student lists the needed equipments for tissue culture laboratory.
LO05 The student could design and describe a tissue culture laboratory.
LO06 The student could outline the needed materials for performing tissue culture.
LO07 The student knows and applies the techniques and conditions relevant to tissue culture.
LO08 The student knows to keep going the tissue culture and keep them in the term of cryopreservation.
LO09 The student distinguishs mitotic cells in tissue culture.
LO10 The student could find the problem and compete with it during tissue culture.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal To make and interpret Medical Genetics, Molecular Genetics and Cytogenetic analyzes 3
PLO02 Bilgi - Kuramsal, Olgusal To have information about genetic diseases 3
PLO03 Bilgi - Kuramsal, Olgusal To have knowledge of Molecular Cell Biology 1
PLO04 Bilgi - Kuramsal, Olgusal To apply fluorescence in-situ hybridization (FISH) technique 1
PLO05 Bilgi - Kuramsal, Olgusal To make DNA isolation and PCR of all kinds of tissue 1
PLO06 Bilgi - Kuramsal, Olgusal To make cell culture of all kinds of tissue (blood, amniotic fluid, chorionic villus biopsy) 4
PLO07 Bilgi - Kuramsal, Olgusal To do cytogenetic analysis by recognizing human chromosomes separately 4
PLO08 Bilgi - Kuramsal, Olgusal To make QF-PCR and to interpret the results 1
PLO09 Bilgi - Kuramsal, Olgusal To have the knowledge and skills to teach Medical Biology and Genetics Courses in Medical Faculties 1
PLO10 Bilgi - Kuramsal, Olgusal To write and implement a research project on his/her own
PLO11 Bilgi - Kuramsal, Olgusal To write an article by collecting the information obtained at the end of the research
PLO12 Yetkinlikler - Öğrenme Yetkinliği To organize laboratory courses of Medical Biology and Genetics courses at undergraduate level 1


Week Plan

Week Topic Preparation Methods
1 What is Tissue culture Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Grup Çalışması
2 Preparation of Tissue culture Laboratory Literature rewiev and reading Öğretim Yöntemleri:
Grup Çalışması
3 Design of Tissue culture Laboratory Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Grup Çalışması
4 Instrumentation used at a tissue culture laboratory Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Grup Çalışması
5 Safety of the tissue culture laboratory Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma
6 Aseptic technique, preparation and sterilization Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma
7 Tissue culture flasks Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
8 Mid-Term Exam Preparing a presentation Ölçme Yöntemleri:
Ödev, Performans Değerlendirmesi
9 Tissue culture media Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
10 Media without sera Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
11 Determination of Mycoplasma contamination and eradication of Mycoplasma Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
12 Primary adherent tissue culture and nonadherent cell lines Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
13 Primary tissue culture Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
14 Cell lines Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
15 Cryopreservation Literature rewiev and reading Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
16 Term Exams Preparing multiple choice and classical tests Ölçme Yöntemleri:
Yazılı Sınav, Performans Değerlendirmesi
17 Term Exams Preparing multiple choice and classical tests Ölçme Yöntemleri:
Yazılı Sınav, Performans Değerlendirmesi


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 4 8 32
Mid-term Exams (Written, Oral, etc.) 1 2 2
Final Exam 1 2 2
Total Workload (Hour) 148
Total Workload / 25 (h) 5,92
ECTS 6 ECTS

Update Time: 23.11.2022 11:29