TÜRKÇE
ENGLISH
Information
| Code | EFR517 |
| Name | |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
It is aimed to explain and applications of molecular biological and biochemical methods used in the investigation of subcellular components and signal transduction system in pharmacology.
Course Content
In the protein measurement, protein expression, measurement of enzyme activity, western-blot, ELISA, polymerase chain reactions used in the investigation of subcellular components and signal transducing systems in molecular pharmacology studies, laboratory safety and basic principles in molecular studies
Course Precondition
None
Resources
Molecular Pharmacology: From DNA to Drug Discovery, Willey-Blackwell, 2013 Principles and Technical Aspect of PCR Amplification, Springer, 2008
Notes
Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 1982
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | List and apply the rules to be considered in laboratory safety and molecular studies. |
| LO02 | Provide sterilization conditions and safe storage of preparations in molecular studies. |
| LO03 | Describe the current methods used in pharmacological studies. |
| LO04 | Relates and interprets methods that can be used in research of subcellular components and signal transduction system in pharmacology experiments. |
| LO05 | Explain and apply molecular methods used in pharmacology research. |
| LO06 | Explain the principle of Western Blot technique, apply and explain in molecular pharmacology research. |
| LO07 | Explain the principle of reverse transcriptase chain reaction technique, apply and explain in molecular pharmacology research. |
| LO08 | Explain the principle of ELISA technique and its use in molecular pharmacology. |
| LO09 | Explain the principle of immunohistochemistry technique and its use in molecular pharmacology. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Explain the mechanisms of action of drugs, their pharmacokinetic and pharmacodynamic effects. | 1 |
| PLO02 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Acts in accordance with ethical and legal principles in the field of pharmacology. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Can project and conduct research in the field of pharmacology and analyze and interpret data. | 3 |
| PLO04 | Yetkinlikler - Alana Özgü Yetkinlik | It plays an active role in rational drug use and provides counseling to the patient for the correct use of the drug. | |
| PLO05 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Can convey the current developments or studies in the field of pharmacology verbally or in writing. | 3 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Follows scientific literature and current developments in the field of pharmacology. | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Establishes a relationship between the effects, mechanisms of action and drug interactions of drugs. | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Explain the physiopathology of various diseases and evalue the drugs used in the treatment of these diseases. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Knows and uses good laboratory practices. | 5 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Uses the theoretical and applied knowledge gained in the field of pharmacology. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Have the ability to take part in in vitro/in vivo animal studies. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction | The subject should be reviewed from the textbooks | |
| 2 | Considerations in Laboratory Safety and Molecular Studies | The subject should be reviewed from the textbooks | |
| 3 | Molecular Pharmacology Studies | The subject should be reviewed from the textbooks | |
| 4 | Methods that can be used in Subcellular Components and Signal Transduction System Research in Pharmacology Experiments | The subject should be reviewed from the textbooks | |
| 5 | Cell culture | The subject should be reviewed from the textbooks | |
| 6 | Preparation of Homogenate from Tissues | The subject should be reviewed from the textbooks | |
| 7 | Protein Measurement Methods | The subject should be reviewed from the textbooks | |
| 8 | Mid-Term Exam | The presentation should be prepare from literature and textbook. | |
| 9 | Basic Principles of Western-Blot Method, Investigation of Protein Expression by Western-Blot | The subject should be reviewed from the textbooks | |
| 10 | Western-Blot Application and Analysis of Data | The subject should be reviewed from the textbooks | |
| 11 | mRNA Analysis by Polymerase Chain Reaction and Reverse Transcriptase Polymerase Chain Reaction | The subject should be reviewed from the textbooks | |
| 12 | Basic Principles of Reverse Transcriptase Polymerase Chain Reaction Technique, Construction, Data Analysis | The subject should be reviewed from the textbooks | |
| 13 | ELISA Method and its use in pharmacological research | The subject should be reviewed from the literature | |
| 14 | Immunohistochemistry and its use in pharmacological research | The subject should be reviewed from the literature | |
| 15 | Student's Presentations and Discussion | Prepare a presentation | |
| 16 | Term Exams | Notes should be studied. | |
| 17 | Term Exams | Notes should be studied. |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 24 | 24 |
| Total Workload (Hour) | 88 | ||
| Total Workload / 25 (h) | 3,52 | ||
| ECTS | 4 ECTS | ||