TÜRKÇE
ENGLISH
Information
| Code | VTS122 |
| Name | Histological Preparation Techniques and Diagnostic Methods |
| Term | 2023-2024 Academic Year |
| Semester | 2. Semester |
| Duration (T+A) | 1-0 (T-A) (17 Week) |
| ECTS | 1 ECTS |
| National Credit | 1 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET HALIGÜR |
| Course Instructor |
Doç. Dr. Mehmet Önder KARAYİĞİT
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
It is aimed to teach the routine techniques and diagnostic methods used in the histological and histopathologic examination of veterinarian candidates working in the laboratory
Course Content
Preparation of histological preparations in the laboratory environment and how to make examinations for preparedness from prepared preparations
Course Precondition
None
Resources
Krause WJ. 2004. The Art of Examining and Interpreting Histologic Preparations, A Laboratory Manual and Study Guide for Histology. Universal Publisher; Lab Manual Edition; Girgin A, Albay B, Liman N, Özfiliz N, Gülmez N, Özcan Z, Yörük M, Erdost H, Aslan Ş, Ergün L, Zık B. 2020. Edit Özer A, Veteriner Özel Histoloji; Lecture notes
Notes
Lecture notes and current literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Identify devices used in histology and pathology and learn the main principles of tissue follow-up |
| LO02 | Learns the detection of tissues and methods of tissue tracking |
| LO03 | Detect and take cross-sections from tailored textures |
| LO04 | Apply histochemical staining techniques to sections |
| LO05 | Prepare a scrotum by learning the scrotum preparation principles |
| LO06 | Learn histochemical staining methods and make these methods |
| LO07 | They can make immunohistochemical staining methods on sections |
| LO08 | They can make histological examination on meat and meat products |
| LO09 | Examine the preparations prepared in light microscope |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns animal species and breeds, recognizes structural, functional and behavioral characteristics (anatomical, histological, physiological, biochemical, etc.). | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | It determines the care and nutrition alternatives for healthy aquaculture and prepares feeding programs suitable for the purpose in accordance with the cultivation patterns and expected yield characteristics of different animal species. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Determine the diagnosis and treatment protocols of diseases. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Learns basic information about chemical and biological substances to be used for prevention and treatment of diseases and their production technologies. | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Evaluates the efficiency of animal breeds. Learns the level of animal breeding. Able to manage an animal selection, hybridization and artificial insemination program. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Makes the most appropriate management planning for livestock enterprises. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | Has knowledge about animal rights and animal owner rights. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has sufficient technology knowledge about hygienic production, production, presentation and sales of animal foods produced for human consumption and / or products of animal products. | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Learns national and international laws and regulations related to veterinary medicine. | |
| PLO10 | Bilgi - Kuramsal, Olgusal | A veterinary practitioner manages animal husbandry enterprises, animal husbandry scientific research centers and animal food production enterprises by utilizing the knowledge gained in the veterinary medicine education and by observing the professional principles. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Recognizes all kinds of diseases that are possible in animals, uses diagnostic methods for this purpose, receives diagnostic materials if necessary and sends them to authorized laboratories and treats the diseases diagnosed by using all methods of medicine (such as interpretation of diagnostic methods results, drug or operative applications). It takes necessary security measures including biosecurity for the control of infectious diseases. | 5 |
| PLO12 | Bilgi - Kuramsal, Olgusal | Prepares and uses the theoretical maintenance-feed protocols. Makes necessary applications for economic aquaculture by considering the characteristics at the business and regional level. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Inspects the production of animal foods and products of animal origin which produced for human consumption and performs health control of the final foodstuffs. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Being aware of the duties and responsibilities to have the consciousness of exercising the profession. Takes responsibility in individual and group work, fulfills the task in line with the skills. | 3 |
| PLO15 | Bilgi - Kuramsal, Olgusal | In cooperation with other experts in problems outside the field of expertise, the team contributes to the solution as a member. Also trying to find solutions to common problems at the regional or national level as well as communication with other professionals. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Comprehends the methods of producing and using scientific knowledge. Evaluates the knowledge and skills gained in a critical way, identifies the new information and information sources that should be acquired in relation to the field and participates in the activities that will meet the learning needs. | 5 |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes tasks related to the field in professional councils and organizations. Based on his professional skills and competences, he plans and realizes social projects with the awareness of society. Provides information on the problems of public and animal health related to the definition of the problem and the solution proposals and organizes events. | 3 |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | He / she can continue his / her professional development and use at least one foreign language (at least European Language Portfolio B1 general level) to establish social relations. At least at the Advanced Level of European Computer Driving License, it uses computer software and other technological tools that may be needed at professional level and social relations level. He follows the world agenda closely and is sensitive to events. | 5 |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Be aware of the needs of the country and the region, and if necessary, guide them in their practice. It defines the subject of human, animal and animal owner rights and considers animal welfare in all its practices. They have the ethical values that are required by the profession, and defend these values when necessary. Have the competence to practice and defense, dominate the concepts of occupational safety in terms of work place and the personnel working with it. It is aware of the environmental health rules and makes practices to protect nature. The environment is sensitive to veterinary public health and zoonoses. | 3 |
| PLO20 | Yetkinlikler - Öğrenme Yetkinliği | Conceptualize the facts and events related to veterinary services; examine scientific techniques and methods, interpret the results; constructs analysis or methods for problems; provides solutions and / or treatment alternatives according to the data obtained. Follows the legislation that is updated on veterinary services and uses them when necessary. | 2 |
| PLO21 | Bilgi - Kuramsal, Olgusal | Evaluate the physiological and physiopathological conditions of animals conceptually and factually. | |
| PLO22 | Yetkinlikler - Alana Özgü Yetkinlik | Evaluates the laboratory results (hematological, biochemical, EKG, EEG, EMG, etc.) of animal diseases and applies correct diagnosis, diagnosis and treatment methods based on the data obtained. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | ntroduction to histological preparation and diagnostic techniques; the purpose of preparation of the preparation, the consumables to be used in preparation of the preparation, the general characteristics of these consumables, the use of materials | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 2 | Microscopes used in histological examination and their properties; classification of microscopes, properties and application areas of light microscope, properties and applications of electron microscopy, properties of immunofluorescence microscopy and application areas | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 3 | Solutions for detection of tissues and detection method; properties of formaldehyde and preparation of buffered formalin solution, properties of alcohols and preparation of alcohols to be used for detection purposes, preparation and application areas of Bouin solution, properties and application areas in methanol | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 4 | Histochemical staining solutions applied to histological sections; hematoxylin preparation and usage areas, eosin preparation and application areas, the staining properties of the tissue and which dye will be painted with the information about | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 5 | The staining used in histological preparations except for routine dyes and their intended use; Properties of dyes used for fixation of inclusion bodies, properties of lengths used for detection of mucinous material | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 6 | Utilization and use of the cathode for immunohistochemical staining; The properties of hyperimmune sera, types of hyperimmune sera, what are the functions of hyperimmune sera, the purpose of use of hyperimmune sera, what are the properties of antibodies used in immunohistochemistry | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 7 | Cryomicrotome tissue sections and fat tissue and cells used for the identification of the staining techniques and general characteristics of dyes, criotom issues to be considered, areas of use of criotom, | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 8 | Mid-Term Exam | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev |
| 9 | Decalcification of bone, decalcification, acquisition of tissue and cross section, | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 10 | Kandan froti hazırlama, frotinin boyanması-incelenme ve değerlendirilmesi, smearın hazırlanması tespit edilmesi ve boyanması, smearin incelenmesinde dikkat edilecek hususlar. | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 11 | Histological analysis methods and their importance in meat products; presence of lung tissue in meat samples, presence of trahea-cartilage-intestine-spleen and similar tissues in meat samples | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 12 | Microscopic examination techniques in meat and meat products; sausage, sausage, sausage examination and reporting, preparing these tissues for microscope, | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 13 | Vaginal smear preparation, staining and examination techniques; The determination of oestrus cycle in female dogs, formation of anoestrus period and the cells seen, the mechanism of the period of diöstrus and the characteristics of the cells seen in this period, the mechanism of the period of proöstrus and the characteristics of the cells seen in this period, the mechanism of the oestrus period and the characteristics of the cells seen during this period | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 14 | Milk somatic cell preparation, staining and examination techniques; somatic cell forms and characteristics, determination of somatic cells in milk, determination of pleomorphic neoplasia cells showing inflammatory or atypia in milk | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 15 | Recognition of cells in inflammatory events; forms and properties of neutrophil leukocytes, forms and properties of eosinophil leukocytes, forms and properties of basophil leukocytes, the shape and properties of plasma cells, the shape and properties of macrophages or histiocytes, what are the roles of these cells in inflammation | lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 16 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev |
| 17 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 1 | 14 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 34 | ||
| Total Workload / 25 (h) | 1,36 | ||
| ECTS | 1 ECTS | ||