BFZ513 Flourescence imaging techniques in the cell

5 ECTS - 3-2 Duration (T+A)- . Semester- 2 National Credit

Information

Code BFZ513
Name Flourescence imaging techniques in the cell
Term 2023-2024 Academic Year
Semester . Semester
Duration (T+A) 3-2 (T-A) (17 Week)
ECTS 5 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Doç. Dr. FİGEN ÇİÇEK


Course Goal / Objective

Learning the fluorescence imaging techniques in the cell and perform fluorescence imaging in the cell.

Course Content

Fluorescence and its physics principles. Chemical fluorescence probes and their cellular usages. Measurement of the basic ions with the fluorescent techniques in the cells Dynamic measurement of the cellular signalling pathways with fluorescent probes. Biological fluorescent probes and their cellular usages. Other techniques; FRET, Fluorescent life time microscopes and immunofluorescent techniques.

Course Precondition

The lesson has no prerequisites

Resources

Lecture notes (For Medical Faculty Students - İsmail Günay) Biophysics (Ferit Pehlivan) Biophysics (Gürbüz Çelebi) Internet Search

Notes

Internet Research


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Learns fluorescence with its physics principles.
LO02 Learns different fluorescence imaging techniques.
LO03 Learns to decide florophore, type of the florescent system, wavelength and filters according to the experimental system.
LO04 Learns image data analysis.
LO05 Ion Imaging in Cell
LO06 Ion imaging in the cell.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Learns basic information about the field 2
PLO02 Bilgi - Kuramsal, Olgusal Sets up and works experimental setups in the laboratory 2
PLO03 Bilgi - Kuramsal, Olgusal Prepares a project proposal on its own
PLO04 Bilgi - Kuramsal, Olgusal Plans an experimental study 2
PLO05 Bilgi - Kuramsal, Olgusal Reports the research result 3
PLO06 Bilgi - Kuramsal, Olgusal Interpret the research findings 2
PLO07 Beceriler - Bilişsel, Uygulamalı Has the analytical thinking mechanism related to the field, Reaches reliable information. Creates and writes a research project, works and researches based on ethical values.
PLO08 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği She/he systematically transfers the theoretical knowledge she has researched to the audience.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Have a scientific thinking systematic in relation to their profession. 3


Week Plan

Week Topic Preparation Methods
1 Basics of the Fluorescence Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Soru-Cevap
2 Biological fluorescence probes and their cellular usages Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Gösteri
3 Chemical fluorescent dyes and their types and usages Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Beyin Fırtınası
4 Fluorescent measurement systems and their working principles 1 Fluorimeters Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Tartışma, Gösteri
5 Fluorescent measurement systems and their working principles 2 Camera and confocal systems Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Gösterip Yaptırma
6 Ion imaging in the cell. Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Tartışma
7 Calibration in the fluorescent measurements Reads the related chapter from the textbook and lecture notes. Öğretim Yöntemleri:
Soru-Cevap, Tartışma
8 Mid-Term Exam Mid-Term Exam Ölçme Yöntemleri:
Yazılı Sınav
9 Fluorescent imaging and Data analysis study Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Tartışma, Beyin Fırtınası
10 Measurement of dynamic ion concentrations in the celli Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Soru-Cevap
11 LASER as a light source Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Beyin Fırtınası, Bireysel Çalışma
12 Fluorescent meaurement of DNA and immunofluorescent techniques Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Benzetim, Problem Çözme
13 FRET and Fluoresence lifetime imaging microscopy Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Soru-Cevap, Tartışma
14 Flow cytometry Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Gösteri, Tartışma
15 A fluorescence system design project work Reads the related chapter from the textbook and lecture notes Öğretim Yöntemleri:
Soru-Cevap, Alıştırma ve Uygulama
16 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 5 5
Final Exam 1 15 15
Total Workload (Hour) 118
Total Workload / 25 (h) 4,72
ECTS 5 ECTS

Update Time: 28.11.2023 02:20