TÜRKÇE
ENGLISH
Information
| Code | BT517 |
| Name | Plant Tissue Culture |
| Term | 2023-2024 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof.Dr. SAADET BÜYÜKALACA |
| Course Instructor |
1 |
Course Goal / Objective
To give general and practical information about plant tissue cultures.
Course Content
Tissue culture-related definitions, techniques used in plant tissue culture, preparation of plant nutrient media, isolation and transferring of plant tissues, culture conditions, acclimatization of in vitro plants to environment, meristem culture, shoot tip culture, cell and callus culture.
Course Precondition
None
Resources
presentations prepared and publications of recent years
Notes
Bitki Biyoteknolojisi (Rüştü Hatipoğlu)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Obtains the conceptual and theoretical knowledge about plant tissue culture. |
| LO02 | Knows the factors affecting the sucsess of plant tissue culture, improvement techniques and methods to increase the success of plant tissue cultures. |
| LO03 | Knows theoretical and practical methods for different researches. |
| LO04 | Knows to transform theoretical knowledge in laboratory studies, experiments. |
| LO05 | Improves new methods to solve present problems. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 4 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | History of plant tissue cultures | Reading recommended books and given lecture notes | |
| 2 | Tissue culture laboratory techniques (preparation of nutrient medium, sterilization techniques of media-explant and equipment used in tissue culture, infection sources in tissue e culture) | Reading recommended books and given lecture notes | |
| 3 | Meristem culture | Reading recommended books and given lecture notes | |
| 4 | Production of disease-free plant | Reading recommended books and given lecture notes | |
| 5 | Embryo culture | Reading recommended books and given lecture notes | |
| 6 | Anther, ovule and microspore culture | Reading recommended books and given lecture notes | |
| 7 | Callus and cell suspension cultures | Reading recommended books and given lecture notes | |
| 8 | Mid-Term Exam | Preparation to mid-term exam | |
| 9 | Direct and indirect organogenesis | Reading recommended books and given lecture notes | |
| 10 | Direct and indirect embryogenesis | Reading recommended books and given lecture notes | |
| 11 | Bioreactors and artificial seed production | Reading recommended books and given lecture notes | |
| 12 | Germplasm preservation | Reading recommended books and given lecture notes | |
| 13 | Somaclonal variations | Reading recommended books and given lecture notes | |
| 14 | Acclimatization of in vitro plants | Reading recommended books and given lecture notes | |
| 15 | General discussion about tissue culture techniques and use of these techniques in Horticulture | Reading recommended books and given lecture notes | |
| 16 | Term Exams | Preparation to final exam | |
| 17 | Term Exams | Preparation to final exam |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||