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Information
| Code | DM0011 |
| Name | Basic Molecular Biology Techniques for Tissue Engineering I |
| Term | 2023-2024 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types, Real Time PCR methods and applications, Blotting techniques, learning application areas (PCR RFLP, Sequence analysis, Gene cloning)
Course Content
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types and Application fields (Mutation screening, PCR RFLP, Sequence analysis, Gene cloning), Real Time PCR methods and applications, Blotting techniques
Course Precondition
None
Resources
Medical Biology and Genetics, Editor: Prof. Dr. Halil Kasap, Nobel Medicine Bookstore, Ankara, 2017
Notes
Pazarbaşı A, Kasap M, Vardar MA, Alptekin D, Güzel AI, Lüleyap HÜ, Altıntaş A, Doran F.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the structure of genetic material |
| LO02 | Knows genetic diseases and genes that cause diseases |
| LO03 | Can perform molecular genetic examinations and analyzes |
| LO04 | It can isolate DNA from any tissue |
| LO05 | To know the basic of PCR |
| LO06 | Perform and interpret agarose and polyacrylamide gel electrophoresis |
| LO07 | Can measure DNA obtained from tissue in spectrophotometer |
| LO08 | Can interpret a scientific article and learn the writing of the article |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Having advanced knowledge of the concepts, theories and principles of the Tissue Engineering Department | 3 |
| PLO02 | Yetkinlikler - Alana Özgü Yetkinlik | Being equipped with advanced mathematics, science and engineering knowledge | |
| PLO03 | Yetkinlikler - Alana Özgü Yetkinlik | To be able to design and perform advanced experiments in the Tissue Engineering Department; To be able to analyze and interpret the results and the data obtained. | 3 |
| PLO04 | Yetkinlikler - Alana Özgü Yetkinlik | Be equipped with a variety of advanced engineering techniques and skills | |
| PLO05 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to lead the definition, design, development and deployment of a product or production method | 2 |
| PLO06 | Yetkinlikler - Alana Özgü Yetkinlik | Being aware of the necessity of lifelong learning and being able to constantly renew oneself | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to use English effectively both verbally and in writing on technical or non-technical subjects | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Being prone to teamwork, being able to communicate well with the environment; Ability to work efficiently as a member of a multi-faceted and interdisciplinary team | 5 |
| PLO09 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Being curious and innovative, aware of its technical and ethical responsibilities | 4 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Gains high-level skills in using research methods in studies related to the field of Tissue Engineering. | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Learning genome analysis methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Mechanism of polymerase chain reaction (PCR) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Optimization of the polymerase chain reaction (PCR) 2 | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | DNA isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Agarose and polyacrylamide gel electrophoresis of PCR products | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | how to prepare solutions and gels and staining gels | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Understanding and applying the QF PCR method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 9 | PCR types | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Analysis and interpretation of PCR and QF PCR results | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Learning Real Time PCR methods and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Blotting techniques | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Application areas (Mutation screening, PCR RFLP) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Sequence analysis | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Gene cloning | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 17 | Final exam | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||