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Information
| Code | VPT 504 |
| Name | Molecular Techniques in Pathology |
| Term | 2023-2024 Academic Year |
| Term | Spring |
| Duration (T+A) | 1-2 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
*What the antigen-antibody is and its storage in the laboratory environment *Antigen-antibody reactions *What are the basic histochemistry and immunohistochemistry methods, *Providing skills about what is PCR WB, ELISA and how to do it.
Course Content
Antigen and antibody structure, material preparation and storage, laboratory use and basic histochemistry and immunohistochemistry procedures. Types and usage areas of electron microscope, working principles of electron microscopes, preparation of tissue pieces for electron microscopy, fixation methods in electron microscopy, tissue tracking, embedding methods, obtaining thin sections, staining methods, examination in electron microscope, evaluation of electron microscopic photographs, PCR, WB, ELISA.
Course Precondition
NONE
Resources
Erer H, Çiftçi MK, Veteriner Genel Patoloji, Bahçıvanlar Basım Sanayi, 2021; Köküuslu C, Genel Patoloji, Medisan yayınevi, 1996; Cheville NF, Introduction to Veterinary Pathology, Iowa State University Press, 1999
Notes
Lecture notes and current literature review
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns how to do molecular techniques used in disease diagnosis |
| LO02 | Gain knowledge of the causes of disease |
| LO03 | Learns the physical factors among the causes of the disease. |
| LO04 | Comprehends the role of chemical causes among disease causes. |
| LO05 | Comprehends the causes of living diseases among the causes of disease and the factors affecting susceptibility and resistance to diseases. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Defines the basic concepts in the field of veterinary pathology, | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Explain species-specific diseases to the field of veterinary pathology | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Defines and uses basic pathology concepts specific to the field of veterinary pathology, | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Learns laboratory procedures and evaluation of samples related to the field of veterinary pathology. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Examines specimens related to veterinary pathology under microscope | 4 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Interprets the results of samples in the field of Veterinary Pathology | 2 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Conducts scientific research to respond to emerging problems in Veterinary, Medicine and health sciences | 3 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Diagnoses the disease in the field of veterinary pathology and ensures that the necessary measures are taken to prevent the disease, | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | It provides the diagnosis of tumors according to animal species in the veterinary field, | 4 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Uses diagnostic methods within the framework of the latest scientific developments in the field of veterinary pathology, | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Designs a field survey to identify animal diseases and zoonotic diseases, determines and manages the sampling method, | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Determines the diagnosis of animal diseases in the field of veterinary pathology and cooperates and consults with various units in the decision-making process in treatment, | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develop critical thinking skills, evaluate evidence critically and make decisions using ways to reach evidence, | 2 |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | It reports data on the prognosis of many types of diseases and tumors detected in the field of veterinary pathology. | 2 |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Provides consultancy using effective communication skills, takes part in teamwork in research, defends scientific ethical rules | |
| PLO16 | Yetkinlikler - Alana Özgü Yetkinlik | Explains the concepts in the field of epidemiology, directs clinical and field research from the beginning, develops methods for determining disease risk factors and disease burdens, provides consultancy in selecting appropriate diagnostic tests, |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Course introduction and application, explanation of evaluation information to the student. Emphasizing the importance of the course in the curriculum and its interaction with other courses. | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | The effects of physical disease causes such as trauma, electric current and heat at the cellular level and their pathological lesions | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Effects of radiation on cells and pathological lesions of physical disease causes | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | Pathological lesions caused by chemical disease causes such as biological toxins and microbial toxins at the cellular level | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Plant toxins, pesticides and herbicides, drug toxicity and pathological lesions caused by metals at the cellular level, which are the causes of chemical diseases. | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | Determination of the factors affecting susceptibility and resistance to diseases. | Preparation of exam questions | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | What viruses and prokaryotic pathogens are and the pathological lesions they cause at the cellular level | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Preparation of exam questions | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 9 | Meanings of terms such as sepsis, septicemia, bacteremia and viremia and the damage caused by these tables at the cell level and determination of these diseases. | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Findings of sepsis, septicemia, viremia and bacteremia in some tissues and organs and detection of these findings in terms of pathology | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Pathological changes in cells caused by disease agents of parasites such as protozoa and metazoa. | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | The effects of nutritional deficiencies on cells and their clinical and pathological manifestations and lesions | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Causes of deficiency of vitamins A, D, E, C, B and C and identification of pathological lesions in cells | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Identification of side effects and pathological lesions of mineral substance deficiencies such as calcium, phosphorus, iron and selenium | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Student presentations and critical giving | Lecture notes and current literature review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Preparation of exam questions | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
| 17 | Term Exams I | Preparation of exam questions | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 86 | ||
| Total Workload / 25 (h) | 3,44 | ||
| ECTS | 3 ECTS | ||