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Information
| Code | BFZ612 |
| Name | Microscopy and Fluorescent Imaging of the Cell |
| Term | 2023-2024 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 7 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. FİGEN ÇİÇEK |
Course Goal / Objective
Learning basic principles of microscopes and decide and choose a proper microscope for a scientific project and use a microscope. Learning fluorescence imaging techniques in the cell and ability to do fluorescence imaging .
Course Content
Basic optic principles, types of microscopes and their working principles. Fluorescent principles and its use in cellular imaging.
Course Precondition
The lesson has no prerequisites
Resources
Lecture notes (For Medical Faculty Students - İsmail Günay) Biophysics (Ferit Pehlivan) Biophysics (Gürbüz Çelebi)
Notes
Internet Research
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns basics of light and optics. |
| LO02 | learns the basic principles of image formation in the Microscope |
| LO03 | Learns the selection criteria of the appropriate microscope for the experiments. |
| LO04 | Learns to use a microscope in the laboratory and image data analysis. |
| LO05 | Learns fluorescence with its physics principles |
| LO06 | Learns different fluorescence imaging techniques |
| LO07 | . Learns to decide florophore, type of the florescent system, wavelength and filters according to the experiment. |
| LO08 | Learns image data analysis. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To learn the basic information about the field | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to establish experimental work in laboratory | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To prepare a project proposal by itself | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To plan an experimental research | |
| PLO05 | Bilgi - Kuramsal, Olgusal | To make a report as a result of the research | 3 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To be able to interpret research findings | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Having analytical thinking mechanism related to the field Accessing reliable information Creating and writing a research project Study and research based on ethic values | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Ability to transfer the theoretical knowledge to the audience in a systematic way | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To have scientific thought systematic about the profession | 4 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Kendi başına bir proje önerisi hazırlayabilmek | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Light and alectromagnetic spectrum | Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Basic optic principles | 1Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Interference, diffraction and resolving power, polarization | 2Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Optical components of the simple microscopes. Magnification and numerical aperture | 3Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Tartışma, Anlatım, Alıştırma ve Uygulama |
| 5 | Lighting techniques in the microscopes (1); bright field, dark field, phase contrast, cross polarized light | 4Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Tartışma, Alıştırma ve Uygulama |
| 6 | Lighting techniques in the microscopes (2); Microspectroscopy (UV and UV-vis) epifluorescence microscopes, confocals | 5Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Gösterip Yaptırma |
| 7 | Other microscope types; Atomic Force, X Ray microscopy, electron microscopy | 6Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Alıştırma ve Uygulama, Soru-Cevap, Anlatım |
| 8 | Mid-Term Exam | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav, Ödev, Performans Değerlendirmesi |
| 9 | Basics of the Fluorescence | 7Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Tartışma, Gösteri |
| 10 | Fluorescence probes and their cellular usages | 8Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 11 | Fluorescent measurement systems and their working principles; Fluorimeter, Camera and confocal systems | 9Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Anlatım |
| 12 | Ion imaging in the cell and calibration | 10Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Soru-Cevap, Alıştırma ve Uygulama, Anlatım |
| 13 | 1Ion imaging in the cell and calibration | 11Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Tartışma, Alıştırma ve Uygulama, Anlatım |
| 14 | Measurement of dynamic ion concentrations in the cell | 12Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Tartışma, Soru-Cevap, Alıştırma ve Uygulama |
| 15 | Fluorescent meaurement of DNA and immunofluorescent techniques | 13Reading the related chapter from the textbooks and notes | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 16 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Ödev, Performans Değerlendirmesi |
| 17 | Term Exams | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Ödev, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 9 | 9 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 173 | ||
| Total Workload / 25 (h) | 6,92 | ||
| ECTS | 7 ECTS | ||