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Information
| Code | ECF421 |
| Name | Pharmaceutical Toxicology Laboratory |
| Term | 2024-2025 Academic Year |
| Semester | 7. Semester |
| Duration (T+A) | 0-3 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 1 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Dr. Öğr. Üyesi Göksun DEMİREL |
| Course Instructor |
Anıl YİRÜN
(A Group)
(Ins. in Charge)
Dr. Öğr. Üyesi Göksun DEMİREL (B Group) (Ins. in Charge) |
Course Goal / Objective
Basic concepts of toxicology; factors affecting toxicity; the fate of xenobiotics in the body; compares the damages caused by drugs and chemical, biological and physical agents to the biological system and the effects of these damages at the macro and molecular level, analyzes and evaluates sufficient theoretical and experimental information so that they can learn to determine harmlessness limits by making toxicological risk assessments.
Course Content
Demonstration (practical approaches in toxicology, classification of poisons according to isolation techniques; isolation techniques of poisons; collection, storage and use of samples; preparation of samples; quantitative and qualitative analysis methods of toxic substances; experimental animals); isolation of acidic and alkaline drugs that cause poisoning by extraction from biological material, identification by primary direct tests and chromatographic methods and evaluation of their toxicity, evaluation of their toxicity by spectrophotometric method.
Course Precondition
None
Resources
1.Klaassen, C.D., Watkins J.W., "Casarett & Doull?s Essential of Toxicology, The McGraw Hill Companies, 2010. 2.Ellenhorn, M.J., "Medical Toxicology", Williams & Wilkins, Maryland, 1997. 3.Hayes, A.W., "Principles and Methods of Toxicology", Taylor & Francis, Philadelphia, 2001
Notes
Demirel G., Yirun A. Toxicology Laboratory Book. Çukurova University, Faculty of Pharmacy Publication No:14.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | In this course, students gain, extraction and analysis of acidic and basic drugs using analytical techniques; determination of volatile substances, metallic poisons, and food additives; DNA isolation; and in vitro cytotoxicity test will be done. |
| LO02 | Compares basic toxicological analyzes. |
| LO03 | Analyzes by extraction of toxic substances from biological samples. |
| LO04 | Analyzes primary direct tests of toxic substances from biological samples. |
| LO05 | Analyzes toxic substances from biological samples by chromatographic method. |
| LO06 | Students run quantitative analysis of toxic substances in the biological samples by spectrophotometric method. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have sufficient knowledge in pharmaceutical field and develop the knowledge. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends professional knowledge with ethical responsibility through current laws and legislation. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Provides consultancy service to patients, community and health care personnel and evaluates advanced knowledge and skills with a critical approach. | 2 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Possessing the knowledge of historical and current developments in the field of Pharmacy, monitoring, evaluation and application of scientific and evidence-based data, and adopting lifelong learning. | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Have the knowledge and skills to help provide patient-oriented pharmacy applications and individualized pharmaceutical care by learning the basics of Clinical Pharmacy, | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes a role in the evaluation and analysis of the events and cases related to the development of pharmacy profession and education. | 3 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Integrates and develops professional knowledge via different disciplines and uses the acquired knowledge to improve the health level and quality of life of the community. | 4 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Use effectively the concepts and applications of basic sciences and basic medical sciences in the field of medicine and pharmacy and update the information. | 4 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates effectively in writing and speaking. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Practices the profession in accordance with Atatürk's principles and reforms, equipped with universal knowledge, uses Turkish correctly and knows at least one foreign language. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Be an example to society withexternal appearance, demeanor, attitude and behavior for the other professionals and society and with professional identity | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Have knowledge about health policies and health economics, pharmacy management and define the problems in pharmaceutical field. | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | Interprets information about current health and treatment problems and attends meeting on related matters . Plays an active role in community health. | 2 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Acquires basic information about diseases, makes decisions about the drugs used in treatment in accordance with rational drug use. | 1 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Demonstration | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım |
| 2 | Isolation of some acide and alkaline drugs from urine sample by liquid-liquid extraction and determination by thin layer chromatography (TLC) | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Isolation and determinaton of some volatile substances from biological sample with microdiffusion technique | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Direct determination of some metalic poisons in biological samples | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Determination of food additives | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Determination of salicylates from serum sample with spectrophotometric assay | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Spectrophotometric determination of paracetamol in plasma | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Lesson Repetition Subject Repetition An overview | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Demostration II | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım |
| 10 | In vitro cytotoxicity test I: MTT analysis | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | In vitro cytotoxicity test II: Trypan Blue Test | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Deney / Laboratuvar |
| 12 | Determination of Ethyl Alcohol in Blood | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 13 | Determination of Methemoglobin in Blood | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Deney / Laboratuvar |
| 14 | Determination of Cyanide in Biological Material by Spectrophotometric Method | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | Determination of Nitrite in Water by Spectrophotometric Method | Reading the Related Section of the Textbook | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Lesson Repetition Subject Repetition An overview | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Lesson Repetition Subject Repetition An overview | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 3 | 3 |
| Final Exam | 1 | 9 | 9 |
| Total Workload (Hour) | 54 | ||
| Total Workload / 25 (h) | 2,16 | ||
| ECTS | 2 ECTS | ||