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Information
| Code | ECS408 |
| Name | Biomolecular Research Methods |
| Term | 2024-2025 Academic Year |
| Semester | 8. Semester |
| Duration (T+A) | 1-1 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 1 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. ÖZLEM ALPTEKİN |
| Course Instructor |
1 2 |
Course Goal / Objective
Students comprehend the basic information about biomolecules and their analysis and gain the ability to decide on method selection in biochemical analysis, which is a part of drug research and development studies.
Course Content
The basic concepts used in the analysis of carbohydrates, proteins, enzymes and nucleic acids, spectrophotometric, electrophoretic and chromatographic lectures are taught to students.
Course Precondition
There is no prerequisite.
Resources
Rodney F, B. Biochemistry laboratory : modern theory and techniques. Pearson Education, Inc. 2012 ISBN 013604302X Görmüş, U. Laboratuvar Dünyası Nobel Kitabevi 2014 ISBN 6053351024 Simpson, R. J . Basic Methods in Protein Purification and Analysis: A Laboratory Manual Cold Spring Harbor Laboratory Press 2008 ISBN 978-0879698676
Notes
Alptekin Ö, lecture notes uploaded to ÇÜBİS system, 2022
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | She/He explains the basic methods used in the analysis of biomolecules. |
| LO02 | She/He can apply some methods used in biomolecules analysis alone. |
| LO03 | She/He can decide which method to use for analysis of biomolecules in a complex situation. |
| LO04 | She/He explains the "Western Blot" technique and its use for proteomic purposes. |
| LO05 | She/He explains the FT-IR technique and its use for proteomic purposes. |
| LO06 | She/He explains spectrophotometric techniques. |
| LO07 | She/He explains recombinant DNA technologies. |
| LO08 | She/He knows DNA diagnostic methods. |
| LO09 | She/He explains DNA diagnosis methods. |
| LO10 | She/He applies the basic steps in protein purification. |
| LO11 | She/He plans and performs enzyme inhibition work. |
| LO12 | She/He evaluates the enzyme inhibition study results and makes the relevant calculations. |
| LO13 | She/He has the ability to conduct advanced research, educate and direct the society in the field of health |
| LO14 | She/ He is competent in lifelong learning, self-renewal and development of pharmaceutical services. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have sufficient knowledge in pharmaceutical field and develop the knowledge. | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends professional knowledge with ethical responsibility through current laws and legislation. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Provides consultancy service to patients, community and health care personnel and evaluates advanced knowledge and skills with a critical approach. | 2 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Possessing the knowledge of historical and current developments in the field of Pharmacy, monitoring, evaluation and application of scientific and evidence-based data, and adopting lifelong learning. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Have the knowledge and skills to help provide patient-oriented pharmacy applications and individualized pharmaceutical care by learning the basics of Clinical Pharmacy, | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes a role in the evaluation and analysis of the events and cases related to the development of pharmacy profession and education. | 2 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Integrates and develops professional knowledge via different disciplines and uses the acquired knowledge to improve the health level and quality of life of the community. | 2 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Use effectively the concepts and applications of basic sciences and basic medical sciences in the field of medicine and pharmacy and update the information. | 5 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates effectively in writing and speaking. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Practices the profession in accordance with Atatürk's principles and reforms, equipped with universal knowledge, uses Turkish correctly and knows at least one foreign language. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Be an example to society withexternal appearance, demeanor, attitude and behavior for the other professionals and society and with professional identity | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Have knowledge about health policies and health economics, pharmacy management and define the problems in pharmaceutical field. | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | Interprets information about current health and treatment problems and attends meeting on related matters . Plays an active role in community health. | 2 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Acquires basic information about diseases, makes decisions about the drugs used in treatment in accordance with rational drug use. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Centrifugation techniques in biochemistry | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Methods used in quantitative determination of proteins. | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Methods for the quantitative determination of nucleic acids. | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Chromatographic techniques used in the purification and analysis of biomolecules | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Spectroscopic analysis of biomolecules | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 6 | Electrophoretic techniques for characterization of proteins and nucleic acids | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | SDS-PAGE application | Relevant experiment protocol is read. | Öğretim Yöntemleri: Soru-Cevap |
| 8 | Mid-Term Exam | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | ELISA techniques and application areas | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Recombinant DNA, molecular cloning and Enzymology | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | DNA techniques in diagnosis I (PCR, ARMS, RFLP, SSCP) | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | DNA techniques in diagnosis II (PCR, ARMS, RFLP, SSCP) | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Protein production, purification and characterization | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Purification of catalase from erythrocytes | Relevant experiment protocol is read. | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar, Bireysel Çalışma |
| 15 | Investigation of the kinetics of catalase in the presence of inhibitors | Relevant experiment protocol is read. | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar, Bireysel Çalışma |
| 16 | Term Exams | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Performans Değerlendirmesi, Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 1 | 14 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 8 | 8 |
| Total Workload (Hour) | 56 | ||
| Total Workload / 25 (h) | 2,24 | ||
| ECTS | 2 ECTS | ||