TÜRKÇE
ENGLISH
Information
| Code | BL317 |
| Name | Moleculer Biology |
| Term | 2024-2025 Academic Year |
| Semester | 5. Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BURHAN ARIKAN |
| Course Instructor |
Prof. Dr. BURHAN ARIKAN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The aim of this course is to teach the structure of nucleic acids in different organisms, mobile genetic structures and isolation, gene cloning, recombinant DNA techniques, PCR and their applications.
Course Content
In this course, introduction and developments in molecular biology, Plasmids and bacteriophages and their properties, Isolation of plasmid DNA and bacteriophage nucleic acids, Characteristics and importance of transposons and transposable structures, DNA isolation from animal and plant cells, Genetic material transfers and methods that occur in microorganisms and plant cells in the natural environment, Electroporation, electrofusion, biolistic transformation methods and applications, Transformation CaCL2, RbCL2, Protoplast and Liposome methods and mechanisms, Gene cloning, PCR Polymerase chain reaction and application areas, Hybridization Sothern, Northern, In situ and Dot blot, Cloning vectors, Recombinant DNA production and transfer to the recipient organism, Molecular typing methods, DNA fingerprint, RFLP, RAPD are covered.
Course Precondition
none
Resources
Recombinant DNA technology, Molecular Biology
Notes
Basic Molecular Biology
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Gene cloning DNA techniques and their application |
| LO02 | Plasmids and Bacteriophages, Isolation |
| LO03 | the mechanisms of natural genetic material of microorganisms in nature |
| LO04 | Understands cloning vectors and their properties |
| LO05 | Conjugation, biyolistic transformation techniques |
| LO06 | Gene cloning and techniques |
| LO07 | Recombinant DNA production carries out its transfer to the recipient organism |
| LO08 | Gene cloning and cloning techniques |
| LO09 | Gasp Polymerase chain reactions |
| LO10 | Hibridisation (Souther, northern, In situ, Dot bloth) |
| LO11 | Makes cloning vectors work |
| LO12 | Recombinant DNA production and transfer to recipient cell |
| LO13 | Isolation and analysis of recombinant organisms |
| LO14 | Hibridoma and cell fusion techniques |
| LO15 | Applies electroporation methods |
| LO16 | Learns to use and applies electrofusion methods |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Explain the basic concepts and principles in biology | |
| PLO02 | Bilgi - Kuramsal, Olgusal | The knowledge gained in the field of biology combine with information from other sciences. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Identifies problems in the field of biology and apply methods for their solution | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Describes the method of producing scientific knowledge in the field of biology | |
| PLO05 | Bilgi - Kuramsal, Olgusal | They will receive information on the biology area, in order to classify it uses and purposes. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | They use advanced theoretical and practical knowledge that obtain in biology field. | |
| PLO07 | Bilgi - Kuramsal, Olgusal | They are Field-related issues can inform the relevant persons and institutions, They share ideas and problems relating to suggestions as the written, oral and Visual to expert or transfer to non-experts. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | They can take responsibility as individuals and team members to solve unpredictable problems encountered in applications related to the field. | 2 |
| PLO09 | Bilgi - Kuramsal, Olgusal | they can take an active role as a member of the execution team the planning of projects related to the field. | 3 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | They know that life-long learning process and act accordingly. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They will increase the knowledge and experience outside major issues with specific elective courses in different fields of biology. | 2 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They speak English enough to communicate with colleagues to understand the basic concepts of biology. | |
| PLO13 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They both with colleagues, as well as areas with non-person establishes ethical uses of information and communication technology. In addition to professional development, in line with the interests and abilities of scientific, social, constantly improves itself by identifying learning needs in cultural and artistic fields. | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | They assesses developments in biology versatile using scientific methods and techniques | |
| PLO15 | Yetkinlikler - Öğrenme Yetkinliği | They know the importance of personal development and enforces the need. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introductions to Molecular Biology | Study in relared section | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 2 | Plasmids and Bacteriophages, Isolation of Plasmid and Bacteriophages nucleic acids | Reading the relevant section | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 3 | Properties and impotance of transposane and transposable structural | Study in topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 4 | DNA isolation of animal and plant cells | free study | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 5 | The mechanisms of natural genetic material of microorganisms and plant cells | Study in literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Deney / Laboratuvar, Bireysel Çalışma |
| 6 | Conjugation, electroporation, electrofusion, biyolistic transformation | Study in section | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Deney / Laboratuvar, Bireysel Çalışma |
| 7 | Transformation CaCL2, RbCL2, Protoplast and Liposome | experimental study | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Researching the relevant area | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 9 | Gene cloning and cloning techniques | Sourch research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 10 | Polymerase chain reactions | topic preparation | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Grup Çalışması, Beyin Fırtınası, Deney / Laboratuvar, Bireysel Çalışma |
| 11 | Hibridisation (Souther, northern, In situ, Dot bloth) | technical work | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Grup Çalışması, Beyin Fırtınası, Deney / Laboratuvar, Bireysel Çalışma |
| 12 | Cloning vectors | Study in related topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Recombinant DNA production and transfer to recipient cell | Free study | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Grup Çalışması, Deney / Laboratuvar, Bireysel Çalışma |
| 14 | Isolation of recombinant organisms | Orientation to research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Grup Çalışması, Deney / Laboratuvar, Bireysel Çalışma |
| 15 | Hibridoma and cell fusion techniques | Study in related research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Studying the sections | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Study of all topics | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 3 | 3 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||