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Information
| Code | BY611 |
| Name | DNA Cloning and Preparation of Gene Libraries |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. HATİCE GÜVENMEZ |
Course Goal / Objective
Comprehend the principles of DNA cloning and forming gene libraries.
Course Content
PCR, basic principles of gene cloning and DNA analysis, Enzymes used in molecular cloning, gene libraries
Course Precondition
none
Resources
Gene Cloning and Analyses of DNA
Notes
Current Literatures
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | understand the pcr and its importance |
| LO02 | comprehend the basic principles of gene cloning and DNA analysis |
| LO03 | comprehend the function of enzymes used in cloning. |
| LO04 | define and compare cloning vectors |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Identifies the interdisciplinary interaction that the field is related to. | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Interprets the knowledge gained in the field by integrating the information from different disciplines and creates new information. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Carries out a study that requires expertise related to the field independently. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Evaluates the knowledge and skills acquired at the level of expertise in the field with a critical approach and directs its learning. | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Supports the current developments in the field and their studies with quantitative and qualitative data and transmits them both in the field and outside the field systematically in written, oral and visual form. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | It supervises the social, scientific, cultural and ethical values in the collection, interpretation, application and announcement stages of the data related to the field and teaches these values. | 4 |
| PLO08 | Bilgi - Kuramsal, Olgusal | The information they absorbed in the field; use problem solving and / or practical skills in interdisciplinary studies. | 4 |
| PLO09 | Belirsiz | It contributes to the solution of social, scientific, cultural and ethical problems encountered in the field and supports the development of these values. | |
| PLO10 | Belirsiz | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Basic principles of gene cloning and DNA analysis. | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | The importance of gene cloning and DNA analysis | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | PCR and its importance | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Örnek Olay |
| 4 | DNA purification from living cells | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Manipulation of purified DNA | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Beyin Fırtınası |
| 6 | DNA entry into living cells | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Vectors for gene cloning | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | An overview | Ölçme Yöntemleri: Sözlü Sınav |
| 9 | Behavior of enzymes such as ligase, polymerase, transferase, transcriptase, endonuclease and exonuclease | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 10 | restriction endonucleases | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 11 | Sequences for recognizing the major class II endonucleases | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Tartışma |
| 12 | The usability of RFI and RFII type supercoil DNA molecules as vector | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım |
| 13 | amplification of genes | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Örnek Olay |
| 14 | Methods for detecting clones | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Gene's Libraries | reading current lecture notes, related papers,and searching internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | An overview | Ölçme Yöntemleri: Sözlü Sınav |
| 17 | Term Exams | An overview | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 4 | 6 | 24 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 10 | 10 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 156 | ||
| Total Workload / 25 (h) | 6,24 | ||
| ECTS | 6 ECTS | ||