TÜRKÇE
ENGLISH
Information
| Code | BK570 |
| Name | Methods for the Diagnosis of Plant Pathogen Bacteria II |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YEŞİM AYSAN |
| Course Instructor |
Prof. Dr. YEŞİM AYSAN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
the aims of the course are learning on identification of pathogenic bacteria, and confirmation of the identification by serological and molecular methods
Course Content
Identification of plant pathogenic bacteria and confirmation of the identification by serological (ELISA, IF, Immunostrip) and molecular methods
Course Precondition
none
Resources
Z. Klement, K. Rudolph,D.C. Sands, 1990. Methods in Phytobacteriology. Akademial Kiodo, Budapest. 567p. Lelliott, R. A., and D. E. Stead, 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Methods in Plant Pathology Volume 2 (Series editor T. F. Preece), Published on behalf of the British Society for Plant Pathology by Blackwell Scientific Publications, Oxford, UK., 219 pages. Saygılı, H., Şahin, F. and Aysan Y., 2006. Fitobakteriyoloji. Meta Basım Matbaacılık, İzmir, 530s.
Notes
Agrios, G.N. 2005. Plant Pathology. Elsevier Academic Press, London, England. pp: 639-647.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | students list tests that support traditional bacterial identification methods. |
| LO02 | Students describe nucleic acid based microbial identification methods. |
| LO03 | Students gain the ability to isolate bacterial DNA. |
| LO04 | Students understand the identification of bacteria by polymerase chain reaction (PCR). |
| LO05 | Students list the steps to determine correlation between bacterial species by multi locus sequence analysis (MLST). |
| LO06 | Students recognize the importance of integration in ibacterial identification . |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Has knowledge on basic topics about general science, general agricultural engineering and plant production | 1 |
| PLO02 | Yetkinlikler - Öğrenme Yetkinliği | Knows the biology, ecology, systematics and control strategies of diseases, pests and weeds causing yield losses in plants | 2 |
| PLO03 | Yetkinlikler - Öğrenme Yetkinliği | Knows the identification and the biology of the diseases and how to control diseases,pests and weeds causing yield losses in plants in practice | |
| PLO04 | Yetkinlikler - Öğrenme Yetkinliği | Comprehends the plant protection problems, gains the ability to solve the problems via scientific methods | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Obtains the skill to use a laboratory, analyzes and evaluates the results and prepares reports | 3 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Decides to the most useful control strategy to cope with the plant protection problems and gets them to be used correctly | 3 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Has necessary knowledge to use the essential technologies, synthesizes them to prepare oral presentations | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | By synthesizing basic and conceptual knowledge about plant protection, she/he gains the ability to develop her/himself in the field of science she/he wants to specialize in, within the framework of the professional qualifications she/he has acquired during her/his undergraduate education | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | He/she can follow and elucidate domestic and international periodicals in the field he/she wants to specialize in. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can present results of the scientific research as a report/thesis and produce scientific publications | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to apply the knowledge gained on environmental/public health and biosecurity issues in relation to agricultural production. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | 116 / 5.000 Çeviri sonuçları Reaches up-to-date resources on plant protection and field and gains the ability to use them for solving problems. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Develops skills in using laboratory methods and techniques | |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Can contribute to the solution of social, scientific, cultural, and ethical problems in the field of plant protection. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Bacterial isolation steps | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Identification of the bacteria by traditional methods | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 3 | Confrimatory tests of traditional identification tests | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 4 | ELISA test in phytobacteriology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 5 | Immunofluorescent (IF) test in phytobacteriology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 6 | Immunostrip test in phytobacteriology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 7 | Bacterial identification by protein and lipopolysaccaride (LPS) anaylsis | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | preparing the exam | Ölçme Yöntemleri: Sözlü Sınav |
| 9 | Bacterial identification by whole cell fatty acid analysis | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 10 | Usage of bacteriophages in phytobacteriology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 11 | Usage of bioluminescent and flow cytometri in phytobacteriology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 12 | Nucleic acid-based microbial identification methods | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 13 | Isolation of bacterial DNA (genomic-DNA) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 14 | Bacterial identification by polymerase chain reaction (PCR) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 15 | genotypic diversity between bacterial species by multi locus sequence analysis (MLST) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | preparing the exam | Ölçme Yöntemleri: Sözlü Sınav |
| 17 | Term Exams | preparing the exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||