TÜRKÇE
ENGLISH
Information
| Code | BT505 |
| Name | Basic Principles of Gene Cloning |
| Term | 2024-2025 Academic Year |
| Term | Fall |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BURHAN ARIKAN |
| Course Instructor |
Prof. Dr. BURHAN ARIKAN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The aim of this course is to teach and practice Gene Cloning and its applications.
Course Content
In this course, the Importance of Gene Cloning, Characteristics and Functions of Plasmids and Bacteriophages, Isolation and Purification of Chromosomal, Plasmid and Bacteriophage DNAs from Living Organisms, Transfer of DNA to Living Organisms Using Transformation Techniques, Characteristics of the Techniques Used and Vectors Used for Cloning Purposes. Topics include polymerase chain reaction, microbiology, gene cloning in agriculture and medicine.
Course Precondition
none
Resources
Gene cloning and DNA analyses
Notes
Gene applications
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | It describes how to evaluate the knowledge and skills acquired at the level of expertise in the field with a critical approach and how to direct one's learning. |
| LO02 | Explains how to systematically convey current developments and own studies in the field to groups in and outside the field in written, verbal and visual form, supported by quantitative and qualitative data. |
| LO03 | Discusses having communication skills in at least one foreign language in order to be able to follow international studies in the field, to take part in various social interactions and scientific study groups at the international level, and to share one's own studies at the international level. |
| LO04 | Discusses the ability to use computer software and information and communication technologies at the level required by the field of biotechnology. |
| LO05 | It describes how to use the knowledge, problem-solving and/or application skills they have acquired in their field in interdisciplinary studies. |
| LO06 | It explains how to deepen the general knowledge of biotechnology science within the framework of basic theories and applications in the field of study and to evaluate the relationships between theories and applications in the field. |
| LO07 | Being aware of social, scientific and ethical values in the studies carried out in the field; categorizes the ability to carry out the research process within the framework of these values. |
| LO08 | Lists the ability to critically evaluate assumptions, ideas and theories related to the field of biotechnology by considering them from a skeptical, logical, analytical and independent perspective. |
| LO09 | Explains how to acquire comprehensive knowledge of modern techniques and methods applied in natural and applied sciences and their limitations |
| LO10 | Describes the ability to develop and deepen knowledge in the field of biotechnology at an expert level. |
| LO11 | Explains how to understand the interdisciplinary interaction that is related to biotechnology. |
| LO12 | Discusses how to interpret and create new knowledge by integrating the knowledge acquired in biotechnology with knowledge from different disciplines. |
| LO13 | Describes how to solve problems encountered in the field using research methods. |
| LO14 | It describes the ability to independently conduct a study that requires expertise in the field. |
| LO15 | Explains how to develop new strategic approaches and take responsibility to produce solutions for unforeseen and complex problems encountered in biotechnology-related applications. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 2 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 4 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 2 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Importance of Gene Cloning, | Study related topics | Öğretim Yöntemleri: Anlatım |
| 2 | Plasmide | Study related topics | Öğretim Yöntemleri: Anlatım |
| 3 | Bakteriophage | Study related topics | Öğretim Yöntemleri: Anlatım |
| 4 | Chromosomal DNA, plasmid and bacteriophage isolation techniques from bacteria | Study related topics | Öğretim Yöntemleri: Beyin Fırtınası |
| 5 | Chromosomal DNA isolation techniques from plants | Study related topics | Öğretim Yöntemleri: Anlatım |
| 6 | Chromosomal DNA isolation methods from yeast | Study related topics | Öğretim Yöntemleri: Anlatım |
| 7 | Chromosomal DNA isolation methods from fungi | Study related topics | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Ödev |
|
| 9 | Conjugation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 10 | Transformation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 11 | Electroporation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 12 | Electrofusion, biolistic transformation and liposome transformation | Study related topics | Öğretim Yöntemleri: Anlatım |
| 13 | Cloning vectors | Study related topics | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Recombinant DNA production and transfer to the recipient organism | Study related topics | Öğretim Yöntemleri: Anlatım, Tartışma |
| 15 | Polymerase chain reaction and applications | Study related topics | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 16 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav, Ödev, Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav, Ödev, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||