TÜRKÇE
ENGLISH
Information
| Code | BT0096 |
| Name | Polymerase Chain Reaction (PCR), display of the Optimization Results |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
Prof. Dr. ARMAĞAN ERDEM ÜTÜK
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The aim of the course is to enable the graduates to have knowledge about Polymerase Chain Reaction, to be able to apply the test and to solve the problems they may encounter during the applications.
Course Content
The components of this course consist of Polymerase Chain Reaction, it's standardisation and the evaluation of the test results.
Course Precondition
NONE
Resources
Lecture notes and presentations will be used on for the courses
Notes
1. Durmaz R, Uygulamalı Moleküler Mikrobiyoloji, 2.baskı, İnönü Üniv.Tıp Fak. Mikrobiyoloji ve Klinik Mikrobiyoloji AD, Malatya, 2001; 149-160. 2. Freeman S, Herron JC, Evrimsel Analiz, Palme Yayıncılık, Ankara, 2009. Lemey P, Salemi M, Vandamme AM, The Phylogenetic Handbook, A Practical Approach to Phylogenetic Analysis and Hypothesis Testing, Cambridge University Press, 2009. 3. Pevsner J, Bioinformatics and Functional Genomics, Wiley-Blackwell, 2009. Xıong J, Essential Bioinformatics, Cambridge University Press, 2006. 4. QiaGen, Critical Factors for Succesful PCR 5. Promega, Product İnformation, Blue/Orange 6X Loading Dye 6. Fermentas, Product İnformation, Protocol for PCR with Taq DNA Polumerase 7. Clip Continental Lab Products, Product İnformation, Model 710 Horizontal Electrophoresis Device
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Applies electrophoresis and imaging procedures for PCR products. |
| LO02 | Optimises the PCR test. |
| LO03 | Lists the PCR and PCR based molecular techniques. |
| LO04 | Describes the DNA ekstraction from the tissues. |
| LO05 | Lists the structure of the cell. |
| LO06 | Solves problems that arise during the PCR application. |
| LO07 | Explains the structure of nucleic acids. |
| LO08 | Explains the stages of replication, transcription, and translation. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 3 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 4 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 2 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Structure of the cell | Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | The structure and replication of of DNA | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 3 | Transcription and translation | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 4 | Gen, Genom and Regulation of DNA | İnternet araştırması yapma Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 5 | Mutations (Genom, Gen and DNA mutations) | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 6 | DNA extraction methods | Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Tartışma, Beyin Fırtınası |
| 7 | Introduction to Polymerase Chain Reaction (PCR) | Textbook reading, Slide review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Studying for an exam | Ölçme Yöntemleri: Sözlü Sınav, Ödev |
| 9 | Modifications of PCR (Semi-Nested PCR, Nested PCR, Multiplex-PCR, RAPDPCR, Real Time-PCR) | Textbook reading, Slide review | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Optimisation in PCR ( enzyme, buffer, magnesium chloride, potassium chloride, dNTP mix, primers) | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 11 | Optimisation in PCR (Optimisation of PCR cycles) | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Beyin Fırtınası |
| 12 | Preparation of gels (agarose, PAGE) that used in the electrophoresis of the amplicons | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 13 | Electrophoresis, staining of PCR gels and screanin of the results | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 14 | Optimisation in the electrophoresis, jel preparation and screening | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 15 | Problems in PCR and solution offers | Slide review, Conducting internet research | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Tartışma, Beyin Fırtınası |
| 16 | Term Exams | Studying for an exam | Ölçme Yöntemleri: Sözlü Sınav, Ödev |
| 17 | Term Exams | Studying for an exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 7 | 98 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 4 | 4 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 1 | 1 |
| Final Exam | 1 | 1 | 1 |
| Total Workload (Hour) | 146 | ||
| Total Workload / 25 (h) | 5,84 | ||
| ECTS | 6 ECTS | ||