TÜRKÇE
ENGLISH
Information
| Code | BT052 |
| Name | Gene Cloning in Microorganisms |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BAHRİ DEVRİM ÖZCAN |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to make students comprehend gene cloning techniques in microorganisms and to understand the biotechnological, agricultural and industrial importance of gene cloning in microorganisms
Course Content
Genome structure of microorganisms, cloning vectors, cloning enzymes, cloning of PCR products, gene transfer methods, yeast cloning vectors, biotechnological, agricultural and industrial importance of gene cloning in microorganisms
Course Precondition
No prerequisite
Resources
Gene Cloning and DNA Analysis, Fifth Edition, 2006, T.A. Brown. Blackwell Publishing, UK. Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999
Notes
Moleküler Biyoteknoloji, Rekombinant DNA'nın Prensipleri ve Uygulamaları, B.R. Glick, C.L. Patten, Beşinci Baskıdan Çeviri, 2021. Çeviri Editörü: T. Kankılıç, Palme Yayınları, Ankara
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the theory of gene cloning techniques in microorganisms |
| LO02 | Isolates a gene from the genomes of microorganisms |
| LO03 | Recognize and use cloning vectors |
| LO04 | Recognize and use DNA modifying enzymes |
| LO05 | Clones a microbial gene |
| LO06 | Knows biotechnological, agricultural and industrial importance of gene cloning in microorganisms |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | 1 |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 2 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 3 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 3 |
| PLO08 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Genome structure of microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 2 | Microbial coning vectors | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 3 | Chromosomal and plasmid DNA isolation from microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 4 | DNA-modifying enzymes and their use in cloning 1 | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 5 | DNA-modifying enzymes and their use in cloning 2 | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 6 | Competent cell preparation from microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 7 | Preparation for mid-term exam | Preparation for mid-term exam | |
| 8 | Mid-Term Exam | Mid-term exams | |
| 9 | Shotgun-based cloning | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 10 | PCR-based cloning | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 11 | Gene transfer methods in microorganisms (transformation, electroporation, protoplast fusion) | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 12 | Selection and analysis of recombinant colonies | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 13 | Biotechnological importance of gene cloning in microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 14 | Agricultural importance of gene cloning in microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 15 | Industrial importance of gene cloning in microorganisms | Molecular Genetics of Bacteria, Edited by JeremyW. Dale, 3th edition, John Wiley and Sons, 1999 | |
| 16 | Term Exams | Term exams | |
| 17 | Term Exams | Term exams |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||