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Information
| Code | ABL581 |
| Name | Experimental Biochemistry |
| Term | 2024-2025 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
Doç. Dr. ÖZLEM ALPTEKİN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
She/He gains an effective understanding of special analysis techniques used in biochemistry, and gain research competence in the field of forensic biochemistry.
Course Content
In this course, basic methods used in biochemistry laboratory are given. The topics that are based on the computer and internet use in biochemistry researches, general laboratory procedures, centrifugation techniques, purification and analysis of biomolecules by chromatographic methods, characterization of proteins and nucleic acids by electrophoresis, spectroscopic analysis of biomolecules, interaction of biomolecules, structure and analysis of nucleic acids, recombinant DNA, molecular cloning and enzymology, purification and characterization of proteins and discussion of current scientific publications are explained.
Course Precondition
There is no prerequisite.
Resources
Switzer, R. L; Garrity, L.F. Experimental biochemistry (theory and exercises in fundamental ), 3. Ed. New York, NY Freeman, 1999.; Boyer, Rodney F. Biochemistry laboratory : modern theory and techniques. 2nd ed. Pearson Education, Inc. 2012
Notes
www.sciencedirect.com; www.scopus.com; www.webofknowledge.com
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | She/ He explains what to pay attention to when working with biological materials. |
| LO02 | She/He recognizes the devices in the laboratory. |
| LO03 | She/He calculates the results of the analysis by considering scientific notation. |
| LO04 | She/He uses her decision-making skills. |
| LO05 | She/He gains research skills. |
| LO06 | She/He recognizes the material and device used in the analysis method. |
| LO07 | She/HeIt counts spectroscopic basis analysis analysis. |
| LO08 | She/He explains the basis of spectroscopic analysis. |
| LO09 | She/He counts the factors affecting the behavior of biological samples in the electrophoretic field. |
| LO10 | She/He explains the basics of electrophoretic techniques. |
| LO11 | She/He explains the basic working principles of instrumental analysis devices used in the analysis of samples. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Knows current concepts in the field of Forensic Sciences. | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends the relations between the areas of Forensic Sciences. | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Comprehends the importance of ethical principles and ethical committees for the individual and society. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows the concept of expertise and forensic procedural approach. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Recognize the statistical methods that are frequently used in studies in the field of Forensic Sciences. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Knows crime scene investigation and evidence collection procedures | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Interprets the knowledge gained in the field by integrating it with the knowledge from different disciplines. | 3 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Uses statistical software that is frequently used in the field of Forensic Sciences. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Uses the knowledge and research methods obtained in the field in solving forensic cases. | 3 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Takes responsibility by participating in teamwork | |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Develops solutions for solving complex problems that may be encountered in the field of Forensic Sciences. | 3 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Can lead in certain challenging Forensic circumstances include interdisciplinary ones. | 3 |
| PLO13 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Follow the scientific meetings and researches in forensic area. | 3 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Discusses current developments and studies with different groups in written, oral and visual formats. | 3 |
| PLO15 | Yetkinlikler - Alana Özgü Yetkinlik | Uses current developments, information and practices in Forensic Sciences for the benefit of society. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Safety in the Laboratory. Keeping Records and Communicating Experimental Results.Using Biochemical Reagents and Solutions. Quantitative Transfer of Liquids. Statistical Analysis of Experimental Data | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Using Computers in Biochemistry. Web Sites Useful in Biochemistry | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | pH, Buffers, Electrodes, and Biosensors. Measurement of Protein Solutions. Measurement of Nucleic Acid Solutions. Techniques for Sample Preparation. Radioisotopes in Biochemistry | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | The centrifugation techniques in biochemistry | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Chromatography and mass spectrometry | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | HPLC method | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Ion exchange chromatography | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Midterm | Studying lecture notes. | Ölçme Yöntemleri: Ödev |
| 9 | Characterization of proteins by electrophoresis | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Nucleic acid purification and characterization by electrophoretic technique | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Spectroscopic analysis of biomolecules | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Biomolecular interactions | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Basic studies of molecular biology | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Diagnostic techniques in DNA | Reading the relevant part of the textbook. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Current literature discussion on studies in biochemistry in Forensic Sciences | Examination of current scientific studies from scientific databases. | Öğretim Yöntemleri: Tartışma |
| 16 | Biochemical calculations | Sample question solution from the relevant textbooks of the topics covered in the course | Öğretim Yöntemleri: Soru-Cevap |
| 17 | Final | Re-studying the topics covered in the course from the relevant textbooks and lecture notes. | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 114 | ||
| Total Workload / 25 (h) | 4,56 | ||
| ECTS | 5 ECTS | ||