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Information
| Code | FİZ602 |
| Name | Methods of intracellular ion measurement: from theory to practise |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. ŞEREF ERDOĞAN |
| Course Instructor |
1 |
Course Goal / Objective
Student learns general intracellular ion measurement methods and comprehensions the microspectrofluorometrıc measurement method especially, measures intracellular ion dynamics by this method and evaluates the findings.
Course Content
General view at intracellular ion measurement methods and inspecting of microspectrofluorometrıc method in details, practicing on this method, measurement of intracellular ion dynamics and evaluation the findings.
Course Precondition
None
Resources
Philips and Baltz; Intracellular ion measurements in single eggs and embryos using ion-sensitive fluorophores. Oxford University Press
Notes
Dagilgan S., Physiology Master Thesis, 2013
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Know to properties of potantiometrical measurement methods, their limitation, advantages and disadvantages |
| LO02 | Explain properties of spectrometrical measurement method, its advantages and disadvantages |
| LO03 | Assume fluorescence ion indicators, their properties and using areas |
| LO04 | Comprehension of error sources for both approaches and properties to correct record |
| LO05 | Make correct and trustful intracellular calcium and pH recordings. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field of physiology with original thought and / or research at the level of expertise gained with the master degree and reach original definitions that will bring innovation to the field. | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction in the field of physiology. Reaches original conclusions using knowledge requiring expertise in analyzing, synthesizing, and evaluating new and complex ideas | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Evaluates uses and analyzes the new information in the field of physiology with a systematic approach. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Develops a new idea, method, design and / or application that brings innovation in the field of physiology or applies a known thought, method, design and / or application to a different area, researches, comprehends, designs and applies an original subject. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Uses the research methods in the field of physiology | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Designs, manages, implements and concludes a study in the field of physiology independently | 1 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Makes scientific research in the field of physiology and / or extends the boundaries of knowledge in the field by producing or interpreting an original work | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Develops new ideas and methods in the field of physiology by using mental processes such as creative and critical thinking, problem solving and decision making. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Keeps up-to-date knowledge by following scientific literature | 2 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Uses the principle of lifelong learning and synthesizes the knowledge in his / her own field and other fields | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops and discusses advanced written, oral and visual communication using at least one foreign language | 3 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Defends the original opinions and communicates effectively with experts | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Introduces scientific, technological, social or cultural advances in the field of physiology and contributes to the process of being and sustaining the society of the society | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Establishes functional interaction by using strategic decision-making processes to solve problems encountered in the field of physiology | |
| PLO15 | Yetkinlikler - Öğrenme Yetkinliği | Contributes to the solution of social, scientific, cultural and ethical problems encountered in the field of physiology and supports the development of these values |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Potantiometrical intracellular ion measurement method, principles based and properties | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Potantiometrical intracellular ion measurement method, its advantages and disadvantages-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Fluorometric intracellular ion measurement method, principles based and properties | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 4 | Fluorometrik intracellular ion measurement method, its advantages and disadvantages-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 5 | Spectrofluorometric method | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 6 | Spectrofluorometric method-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 7 | Microspectrofluorometric measurement method | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Review of lessons learned | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
| 9 | Microspectrofluorometric measurement method-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Gösterip Yaptırma |
| 10 | Fluorescence indicators and their properties | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama |
| 11 | Fluorescence indicators and their properties-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Gösterip Yaptırma |
| 12 | Intracellular calcium measurement | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Alıştırma ve Uygulama, Gösterip Yaptırma |
| 13 | Intracellular calcium measurement-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Deney / Laboratuvar |
| 14 | Intracellular pH measurement | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Alıştırma ve Uygulama, Gösterip Yaptırma |
| 15 | Intracellular pH measurement-2 | Study of subject from advised sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösterip Yaptırma, Deney / Laboratuvar |
| 16 | Term Exams | Review of lessons learned | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | Review of lessons learned | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 3 | 3 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||