TÜRKÇE
ENGLISH
Information
| Code | FİZ602 |
| Name | Methods of intracellular ion measurement: from theory to practise |
| Term | 2000-2001 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. ŞEREF ERDOĞAN |
| Course Instructor |
1 |
Course Goal / Objective
Student learns general intracellular ion measurement methods and comprehensions the microspectrofluorometrıc measurement method especially, measures intracellular ion dynamics by this method and evaluates the findings.
Course Content
General view at intracellular ion measurement methods and inspecting of microspectrofluorometrıc method in details, practicing on this method, measurement of intracellular ion dynamics and evaluation the findings.
Course Precondition
None
Resources
Philips and Baltz; Intracellular ion measurements in single eggs and embryos using ion-sensitive fluorophores. Oxford University Press
Notes
Dagilgan S., Physiology Master Thesis, 2013
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Know to properties of potantiometrical measurement methods, their limitation, advantages and disadvantages |
| LO02 | Explain properties of spectrometrical measurement method, its advantages and disadvantages |
| LO03 | Assume fluorescence ion indicators, their properties and using areas |
| LO04 | Comprehension of error sources for both approaches and properties to correct record |
| LO05 | Make correct and trustful intracellular calcium and pH recordings. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Potantiometrical intracellular ion measurement method, principles based and properties | Study of subject from advised sources | |
| 2 | Potantiometrical intracellular ion measurement method, its advantages and disadvantages-2 | Study of subject from advised sources | |
| 3 | Fluorometric intracellular ion measurement method, principles based and properties | Study of subject from advised sources | |
| 4 | Fluorometrik intracellular ion measurement method, its advantages and disadvantages-2 | Study of subject from advised sources | |
| 5 | Spectrofluorometric method | Study of subject from advised sources | |
| 6 | Spectrofluorometric method-2 | Study of subject from advised sources | |
| 7 | Microspectrofluorometric measurement method | Study of subject from advised sources | |
| 8 | Mid-Term Exam | Review of lessons learned | |
| 9 | Microspectrofluorometric measurement method-2 | Study of subject from advised sources | |
| 10 | Fluorescence indicators and their properties | Study of subject from advised sources | |
| 11 | Fluorescence indicators and their properties-2 | Study of subject from advised sources | |
| 12 | Intracellular calcium measurement | Study of subject from advised sources | |
| 13 | Intracellular calcium measurement-2 | Study of subject from advised sources | |
| 14 | Intracellular pH measurement | Study of subject from advised sources | |
| 15 | Intracellular pH measurement-2 | Study of subject from advised sources | |
| 16 | Term Exams | Review of lessons learned | |
| 17 | Term Exams | Review of lessons learned |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 3 | 3 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 125 | ||
| Total Workload / 25 (h) | 5,00 | ||
| ECTS | 5 ECTS | ||