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Information
| Code | BİO605 |
| Name | Molecular Genetics (Z) |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. HÜSNÜ ÜMİT LÜLEYAP |
Course Goal / Objective
To give the necessary information for understanding the structure, organization and functions of prokaryotic and eukaryotic genetic material in the normal life of living organism and its importance in some diseases.
Course Content
Introduction, basic concepts of molecular genetics. Eukaryotic genome organization and DNA sequences. Packaging of DNA: Chromatin and Chromosome. Whole genome sequence defined prokaryotic and eukaryotic genomes. Bioinformatics and System Biology. Replication of DNA. Telomer and telomerase. Repair of DNA. Homologous sequence recombination. Transpositions and DNA amplification. Transcription in prokaryotes; Eukaryotic RNA polymerases and transcription factors. Transcription activators and repressors. Chromatin structure and transcription. Regulation of transcription in eukaryotes; Promotor and enhancers. Proteins that regulate transcription. Processing and cycle of RNA: processing of rRNA, tRNA and mRNA. Splicing mechanisms, alternative splycing, RNA editing, RNA degradation, Translation of mRNA. Bending and processing of proteins. Regulation of protein functions. Midterm /final exam
Course Precondition
Must have taken BIO-501 Medical Biology and BIO-502 Medical Genetics courses
Resources
1-Moleküler Genetiğin Esasları,, Ümit LÜLEYAP , Akademisyen Kitabevi. 2. Baskı 2020 2-Molecular Biology of the cell. Bruce Alberts Garland Publishing. 2008
Notes
1-Moleküler Genetiğin Esasları,, Ümit LÜLEYAP , Akademisyen Kitabevi. 2. Baskı 2020 2-Molecular Biology of the cell. Bruce Alberts Garland Publishing. 2008
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows the basic concepts of molecular genetics |
| LO02 | is able to establish the relationship gene, genome, chromatin and chromosome |
| LO03 | is able to compare gene sequences and genome organization between prokaryotes and eukaryotes and to interpret evolutionary developments |
| LO04 | is able to explain the reproduction, mutation and mutation repair mechanisms of genomic DNA. |
| LO05 | is able to distinguish and explain programmed and random changes (recombination and rearrangement) between homologous, non-homologous DNA sequences |
| LO06 | is able to explain the transcription of RNA in prokaryotes and eukaryotes, control of transcription process, processing and transformation of RNA |
| LO07 | is able to explain the expression of genetic code as protein |
| LO08 | is able to list functional processes of synthesized proteins |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To make and interpret Medical Genetics, Molecular Genetics and Cytogenetic analyzes | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To have information about genetic diseases | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To have knowledge of Molecular Cell Biology | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To apply fluorescence in-situ hybridization (FISH) technique | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To make DNA isolation and PCR of all kinds of tissue | 4 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To make cell culture of all kinds of tissue (blood, amniotic fluid, chorionic villus biopsy) | |
| PLO07 | Bilgi - Kuramsal, Olgusal | To do cytogenetic analysis by recognizing human chromosomes separately | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To make QF-PCR and to interpret the results | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To have the knowledge and skills to teach Medical Biology and Genetics Courses in Medical Faculties | 2 |
| PLO10 | Bilgi - Kuramsal, Olgusal | To write and implement a research project on his/her own | 2 |
| PLO11 | Bilgi - Kuramsal, Olgusal | To write an article by collecting the information obtained at the end of the research | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To organize laboratory courses of Medical Biology and Genetics courses at undergraduate level |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction, basic concepts of molecular genetics, eukaryotic genome organization and DNA sequences. | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Packaging of DNA: Chromatin and Chromosome | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Whole genome sequence defined prokaryotic and eukaryotic genomes, Bioinformatics and System Biology | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Replication of DNA | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 5 | Repair of DNA | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | Homologous sequence recombination | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Transpositions and DNA amplification | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 8 | Mid-Term Exam | Preparation of multiple-choice questions | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Transcription in prokaryotes; Eukaryotic RNA polymerases and transcription factors | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | . Transcription activators and repressors. Chromatin structure and transcription. | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 11 | Regulation of transcription in eukaryotes; Promotor and enhancers | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 12 | Proteins that regulate transcription. | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Processing and cycle of RNA: processing of rRNA, tRNA and mRNA | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Splicing mechanisms, alternative splycing, RNA editing, RNA degradation | Slide preparation | Öğretim Yöntemleri: Tartışma |
| 15 | Translation of mRNA. Folding and processing of proteins. Regulation of protein functions | Slide preparation | Öğretim Yöntemleri: Anlatım, Tartışma |
| 16 | Term Exams | Preparation of multiple-choice questions | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation of multiple-choice questions | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 4 | 8 | 32 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 2 | 2 |
| Total Workload (Hour) | 148 | ||
| Total Workload / 25 (h) | 5,92 | ||
| ECTS | 6 ECTS | ||