TB607 Laboratory Techniques in Biotechnology

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code TB607
Name Laboratory Techniques in Biotechnology
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal / Objective

The aim of this course is to provide theoretical information and practice on working techniques in biotechnology laboratories and biotechnological methods used in plants.

Course Content

536 / 5.000 This course covers the explanation of the concepts of biotechnology, plant biotechnology, in vitro culture, introduction of the equipment of the biotechnology laboratory, nutrient media used in in vitro culture, preparation of macro and micro element stock solutions, preparation of growth regulator and vitamin stock solutions, sterilization principles of glassware and metal materials, preparation of nutrient media, sterilization of plant material, embryo culture, anther culture, DNA isolation, PCR analysis.

Course Precondition

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Resources

Hatipoğlu, R. 1993. Biyoteknolojide Laboratuar teknikleri ders notu

Notes

Hatipoğlu, R. 1993. Biyoteknolojide Laboratuar teknikleri ders notu


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Knows the concepts of biotechnology, plant biotechnology, in vitro culture, genetic engineering, molecular markers
LO02 Have knowledge about the infrastructure and equipment required in a biotechnology laboratory.
LO03 Can apply topics such as preparation of nutrient medium, sterile work, some in vitro culture techniques, DNA isolation, PCR, etc.
LO04 Has knowledge of sterile work and safety precautions


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Graduates become a specialist on the field crops area by improving their skills. 1
PLO02 Bilgi - Kuramsal, Olgusal They comprehend interdisciplinary interaction in his specialization area.
PLO03 Beceriler - Bilişsel, Uygulamalı They can follow the latest developments in field crops area and get access to the knowledge source, gather this knowledge, reach new knowledge and synthesize by evaluating available studies and follow the innovations in his field. 2
PLO04 Beceriler - Bilişsel, Uygulamalı They can synthesize and interpret interdisciplinary knowledge by using theoretical and practical skills at a specialist level in field crops area. 3
PLO05 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They find solutions for problems related to field crops by using research methods and establish cause effect relationships.
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They incorporate their knowledge on his special area by combining their knowledge with those from the other scientific areas and produce new knowledge and also solve the problems by using scientific research methods. 5
PLO07 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They determine a problem independently in field crops subject, provide solutions, evaluate the results and implement whenever required.
PLO08 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They are qualified to solve a problem in their field.
PLO09 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They are equipped with analytical and critical thinking ability to direct their learning and to conduct advanced studies in their fields independently.
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They transfer current developments and their own studies in the field crops area systematically to the his working group and to different groups from other study fields orally or visually by supporting them with qualitative and quantitive data. 4
PLO11 Yetkinlikler - İletişim ve Sosyal Yetkinlik They study and improve the social relationships and standards leading these relationship by a critical point of view and take action when needed.
PLO12 Yetkinlikler - Alana Özgü Yetkinlik They are able to understand and translate an article written in a foreign language.
PLO13 Yetkinlikler - Alana Özgü Yetkinlik They develop policy, strategy and experimental plans related to field crops area and evaluate obtained results within the framework of quality processes.


Week Plan

Week Topic Preparation Methods
1 Explanation of Biotechnology, Plant Biotechnology, and In Vitro Culture Concepts Pre-reading Öğretim Yöntemleri:
Anlatım
2 Introducing Biotechnology Laboratory Equipment Pre-reading Öğretim Yöntemleri:
Anlatım
3 Nutrient Media for In Vitro Culture Pre-reading Öğretim Yöntemleri:
Anlatım
4 Preparing Macro and Microelement Stock Solutions Pre-reading Öğretim Yöntemleri:
Anlatım
5 Preparing Growth Regulator and Vitamin Stock Solutions Pre-reading Öğretim Yöntemleri:
Anlatım
6 Principles of Sterilizing Glassware and Metalware Pre-reading Öğretim Yöntemleri:
Anlatım
7 Nutrient Media Preparation Pre-reading Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam Pre-reading Ölçme Yöntemleri:
Yazılı Sınav
9 Principles of Working in a Sterile Cabin Pre-reading Öğretim Yöntemleri:
Anlatım
10 Sterilization of Plant Material: A Homework Assignment Will Be Given Pre-reading Öğretim Yöntemleri:
Anlatım
11 Embryo culture Pre-reading Öğretim Yöntemleri:
Anlatım
12 Anther culture Pre-reading Öğretim Yöntemleri:
Anlatım
13 DNA Isolation Pre-reading Öğretim Yöntemleri:
Anlatım
14 PCR analysis of DNA with pre-determined quality and quantity Pre-reading Öğretim Yöntemleri:
Anlatım
15 Analyzing PCR Results Pre-reading Öğretim Yöntemleri:
Anlatım
16 Term Exams Pre-reading Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Pre-reading Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 0 0
Mid-term Exams (Written, Oral, etc.) 1 8 8
Final Exam 1 24 24
Total Workload (Hour) 144
Total Workload / 25 (h) 5,76
ECTS 6 ECTS

Update Time: 03.10.2024 01:55