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Information
| Code | BİO527 |
| Name | Fluorescence In Situ Hibdiridizasyon (FISH) Theoretical and Practical Application |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYFER PAZARBAŞI |
Course Goal / Objective
Presenting analyses method, application fields, mechanism, characteristics of used probes, usage fields and routine applications of FISH technique.
Course Content
FISH Analyses method, Application fields of FISH technique, The mechanism of FISH technique, NISH, FISH, Characteristics of used probes, Probe marking methods, Hybridization conditions, Visualization systems, Usage fields, Routine applications of FISH, Diagnosis of translocations and numerical abnormalities at leukemia genetics, Diagnosis of structural and numerical abnormalities with FISH techniques at cancer genetics, Diagnosis of aneuploidies with FISH techniques at preimplantation genetics, FISH applications at reproductive pathologies.
Course Precondition
To have taken the BIO 525 CYTOGENETICS course
Resources
Methods in Human Cytogenetics
Notes
FISH applications
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The student defines FISH Analyses method |
| LO02 | The student lists application fields of FISH technique |
| LO03 | The student explains The mechanism of FISH technique |
| LO04 | The student explains NISH, FISH terms |
| LO05 | The student lists Characteristics of used probes |
| LO06 | The student tells Probe marking methods |
| LO07 | The student expresses Hybridization conditions |
| LO08 | The student knows Visualization systems |
| LO09 | The student expresses Usage fields |
| LO10 | The student tells routine applications of FISH |
| LO11 | The student tells diagnosis of translocations and numerical abnormalities at leukemia genetics |
| LO12 | The student expresses diagnosis of structural and numerical abnormalities with FISH techniques at cancer genetics |
| LO13 | The student expresses diagnosis of aneuploidies with FISH techniques at preimplantation genetics |
| LO14 | The student expresses FISH applications at reproductive pathologies |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To comprehend the structure and functions of cells and organelles which constitute the basic structure of life | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To recognize the functions of tissue and organ systems | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To examine the structure of genetic material | 5 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To recognize genetic diseases and genes that cause these diseases | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To learn the structure of genes | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To define chromosome and gene mutations | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To classify heredity forms (autosomal dominant, autosomal recessive, gender-dependent dominant and recessive inheritance models) | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To use molecular and cytogenetic analysis | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To be able to explain the numerical and structural anomalies of autosomal and sex chromosomes | 3 |
| PLO10 | Bilgi - Kuramsal, Olgusal | To make DNA isolation from all kinds of tissues | 4 |
| PLO11 | Bilgi - Kuramsal, Olgusal | To prepare PCR | 4 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To make and interpret agarose and polyacrylamide gel electrophoresis | 2 |
| PLO13 | Bilgi - Kuramsal, Olgusal | To measure the obtained DNA on the spectrophotometer | 4 |
| PLO14 | Bilgi - Kuramsal, Olgusal | To apply and interpret QF-PCR technique | 2 |
| PLO15 | Bilgi - Kuramsal, Olgusal | To make cell culture from blood, amnion, chorionic villus biopsy specimens and to obtain chromosome preparation | |
| PLO16 | Bilgi - Kuramsal, Olgusal | To perform karyotype analysis of chromosome preparations and to recognize and interpret all human chromosomes | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | To interpret a scientific article and to learn how to write a paper |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | FISH Analyses method | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 2 | Application fields of FISH technique | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 3 | The mechanism of FISH technique | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 4 | NISH, FISH | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 5 | Characteristics of used probes | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 6 | Probe marking method | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 7 | Hybridization conditions | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparing a presentation | Öğretim Yöntemleri: Beyin Fırtınası |
| 9 | Visualization systems | Literature review and reading | Öğretim Yöntemleri: Tartışma |
| 10 | Usage fields | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 11 | Routine applications of FISH | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 12 | Diagnosis of translocations and numerical abnormalities at leukemia genetics | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 13 | Diagnosis of structural and numerical abnormalities with FISH techniques at cancer genetics | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 14 | Diagnosis of aneuploidies with FISH techniques at preimplantation genetics | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 15 | FISH applications at reproductive pathologies | Literature review and reading | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Preparing multiple choice and classicAL TESTS | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 6 | 6 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 126 | ||
| Total Workload / 25 (h) | 5,04 | ||
| ECTS | 5 ECTS | ||