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Information
| Code | Trans605 |
| Name | Translational Laboratory Techniques I |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 1-4 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | İngilizce |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types, Real Time PCR methods and applications, Blotting techniques, learning application areas (PCR RFLP, Sequence analysis, Gene cloning)
Course Content
Genome analysis methods, Polymerase chain reaction (PCR) mechanism and optimization, DNA isolation, Agarose and polyacrylamide gel electrophoresis of PCR products, Preparation of solutions and gels and staining of gels, QF PCR method, analysis and interpretation of PCR and QF PCR results, PCR types and Application fields (Mutation screening, PCR RFLP, Sequence analysis, Gene cloning), Real Time PCR methods and applications, Blotting techniques
Course Precondition
Basic molecular biology knowledge is required
Resources
Medical Biology and Genetics, Editor: Prof. Dr. Halil Kasap, Akademisyen Bookstore, 4th edition, 2020
Notes
Pazarbaşı A, Kasap M, Vardar MA, Alptekin D, Güzel AI, Lüleyap HÜ, Altıntaş A, Doran F. Screening of Codon 236, 238, 244, 248 and 259 Mutations of p53 Gene in Ovarian and Endometrial Carcinomas by RFLP Analysis. Ç.Ü.Sağlık Bil.Derg. 2007,23(2,3):1-8.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows genetic diseases and genes that cause diseases |
| LO02 | Knows the structure of genetic material |
| LO03 | Can perform molecular genetic examinations and analyzes |
| LO04 | It can isolate DNA from any tissue |
| LO05 | To know the basic of PCR |
| LO06 | Perform and interpret agarose and polyacrylamide gel electrophoresis |
| LO07 | Can measure DNA obtained from tissue in spectrophotometer |
| LO08 | Can interpret a scientific article and learn the writing of the article |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops and deepens the current and advanced level knowledge of translational medicine via unique thoughts or researches at a level of proficiency, find out original definitions that will bring innovation in the field of translational medicine. | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Conceives the interdisciplinary interactions related to translational medicine; analyzes synthesizes and evaluates original and new thoughts. | 5 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Explains the usage of tool, devices and instruments requiered for knowledge and technologies about translational medicine and its related disciplines. | 5 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Defines frequently used statistical methods in translational medicine and related disciplines, uses statistical softwares effectively. | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Uses both theoretical and practical knowledge at an advanced level in the studies related to translational medicine. | 5 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Develops a new thoughts, method and designment/application which brings innovation in translational medicine or implements a known thoughts, method and designment/application in different fields, investigates, comprehends, designs, adapts, implements an original topic. | 5 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Writes the report of his/her research which he/she participated, | 5 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Makes necessary investigations by using tool, devices and instrument required for knowledge and technologies about translational medicine and related disciplines at an advanced level, develops a new and creative solution (device, method, treatment, drug) for the problems. | 5 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Uses statistical software in the field of Translational Medicine effectively, chooses statistical methods correctly, calculates and interprets correctly. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Maintains the organization of Translational Medicine laboratories and develops solutions in case of encountering unforeseen complex situations during laboratory working hours. | 5 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific studies in translational medicine and related fields independently or as a team member. | 5 |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Writes the report of his/her research in which he/she participated, publishes in a national or international reputed journal (indexing in SCI, SCI-Expanded, SSCI, or AHCI), and presents it at scientific meetings. | 5 |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Follows evidence-based practices and conducts research on professional practices that will create evidence in their field. | 5 |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | Applies the principles of advanced professional development and lifelong learning in the field of Translational Medicine. | 5 |
| PLO15 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicate current developments and studies within the field to both professional and non-professional groups systematically using written, oral and visual techniques by supporting with quantitative and qualitative data. | 5 |
| PLO16 | Bilgi - Kuramsal, Olgusal | Learns how to teach a concept, subject, phenomenon or case related to Translational Medicine. | |
| PLO17 | Belirsiz | Communicate and discuss orally, in written and visually with peers by using a foreign language at least at a level of European Language Portfolio B2 General Level. | |
| PLO18 | Yetkinlikler - Alana Özgü Yetkinlik | Audit the data gathering, interpretation, implementation and announcement stages by taking into consideration the cultural, scientific, and ethic values and uses these issues for social strategy, implementation plans and frame of quality processes. | |
| PLO19 | Yetkinlikler - Alana Özgü Yetkinlik | Knows the importance of ethical principles and ethical committees for the individual and society, acts ethically. | |
| PLO20 | Yetkinlikler - Alana Özgü Yetkinlik | Increases the awareness of society about translational medicine. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Learning genome analysis methods | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Mechanism of polymerase chain reaction (PCR) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Optimization of the polymerase chain reaction (PCR) 2 | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | DNA isolation | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Agarose and polyacrylamide gel electrophoresis of PCR products | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | How to prepare solutions and gels and staining gels | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Understanding and applying the QF PCR method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
|
| 9 | PCR types | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Analysis and interpretation of PCR and QF PCR results | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Learning Real Time PCR methods and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Blotting techniques | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Application areas (Mutation screening, PCR RFLP) | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Sequence analysis | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Gene cloning | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
| 17 | Term Exams | Literature browsing and reading | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 128 | ||
| Total Workload / 25 (h) | 5,12 | ||
| ECTS | 5 ECTS | ||