TÜRKÇE
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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| MEDICAL BIOCHEMISTRY (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | BİK581 |
| Name | LABORATORY TECHNIQUES AND METHODS I |
| Term | 2025-2026 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is to provide students with both theoretical knowledge and practical skills related to fundamental laboratory techniques used in scientific research and diagnostics. The course focuses on proper use of laboratory equipment, preparation of solutions, sample handling, data interpretation, and adherence to safety and ethical standards in laboratory environments. Through hands-on experience and critical analysis, students will enhance their problem-solving abilities and develop professional laboratory competence.
Course Content
Introduction to Laboratory Safety, Hazardous Materials, and Ethical Guidelines 2 Basic Laboratory Equipment and Instrumentation (balances, heaters, glassware) 3 Preparation of Solutions: Molarity, Normality, and Dilution Calculations 4 Pipetting Techniques: Manual and Micropipette Applications 5 pH Measurement and Use of pH Meters; Buffer Systems 6 Centrifugation: Principles and Applications 7 Spectrophotometric Measurements and Calibration Curve Preparation 8 Introduction to Electrochemical Methods: Conductivity Measurements
Course Precondition
be successful in the exam
Resources
Lehninger ve lippincot
Notes
Harvey, D. (2000). Modern Analytical Chemistry. McGraw-Hill. Skoog, D.A. et al. Fundamentals of Analytical Chemistry. Dean, J.A. Analytical Chemistry Handbook.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Describe laboratory safety rules, chemical hazard classifications, and ethical conduct. |
| LO02 | Prepare standard and buffer solutions with appropriate calculations (molarity, normality, dilutions) |
| LO03 | Apply essential techniques such as pipetting, centrifugation, and spectrophotometry. |
| LO04 | Design simple experiments and contribute to method development processes. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Explain the structure and function of the basic tissues of the body. | 1 |
| PLO02 | Belirsiz | Explain the structure and functions of the cell, their physiological mechanisms and bioelectrical events. | 1 |
| PLO03 | Belirsiz | Explain the structure and functions of biochemical molecules involved in metabolism. | 1 |
| PLO04 | Belirsiz | Know the structure and function of genetic material (DNA and RNA), gene expression, genome structure, nucleic acid metabolism and the use of DNA in medicine, the basic principles of heredity. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to Laboratory Safety, Hazardous Materials, and Ethical Guidelines | Think of a chemical hazard you’ve encountered at home or school and be ready to discuss it in class. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Basic Laboratory Equipment and Instrumentation (balances, heaters, glassware) | Choose 3 lab instruments; research where they are used and how they operate. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Study solution concentrations: molarity, normality, and dilution calculations. | Identify real-life contexts (e.g., pharmacy, kitchen) where solution preparation is applied. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Farklı pipet türlerini ve pipetleme tekniklerini karşılaştırınız. | Reflect on how pipetting is prone to human error. What precautions should be taken? | Öğretim Yöntemleri: Anlatım, Gösteri |
| 5 | Learn the concept of pH and how buffer solutions work. | Find 2 real-life cases where pH is crucial (e.g., stomach acid, shampoo formulation). | Öğretim Yöntemleri: Anlatım |
| 6 | Microarray and Genomic Analyses | Microarray technology, bioinformatics tools, gene expression, and genomic analysis. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 7 | PCR and Real-Time PCR Techniques | PCR, RT-PCR, and qPCR techniques for genetic analysis, gene expression analysis. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Sözlü Sınav |
|
| 9 | Study how centrifugation is used in blood testing or cell separation procedures. | Study the working principle of a centrifuge and types of rotors. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Read about the basic working principle of spectrophotometers and the absorbance-concentration relationship. | Study how centrifugation is used in blood testing or cell separation procedures. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Molecular Cloning and Genetic Modification Techniques | Genetic engineering applications: Gene cloning, PCR, restriction enzymes, ligation techniques. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Biotechnological Applications and Genetic Modification | CRISPR-Cas9 technology, genetic engineering, and biotechnological product production. | Öğretim Yöntemleri: Soru-Cevap, Alıştırma ve Uygulama |
| 13 | Enzyme and Protein Mutations | Protein engineering, modification of enzyme functions, mutagenesis techniques. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 14 | Data Analysis and Result Interpretation | Laboratory data analysis, statistical methods, and interpretation of biological results. | Öğretim Yöntemleri: Soru-Cevap, Alıştırma ve Uygulama |
| 15 | Advanced Protein Structural Analyses | Protein structure analysis using X-ray crystallography, NMR, and cryo-EM techniques. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||