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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| MEDICAL BIOCHEMISTRY (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | BİK582 |
| Name | LABORATORY TECHNIQUES AND METHODS II |
| Term | 2025-2026 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-4 (T-A) (17 Week) |
| ECTS | 7 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To be able to perform medical analyses accurately in laboratories for purposes such as diagnosis, treatment monitoring and health screening.
Course Content
Laboratory safety and ethical guidelines Protein isolation and proteomics analysis Spectroscopic methods (UV-Vis, FTIR, NMR) PCR and Real-Time PCR techniques Microarray and genomic analyses Mass spectrometry and proteomics analysis Enzyme kinetics and enzymatic reactions Molecular cloning and genetic modification techniques Cellular imaging and microscopy techniques Genetic modification in biotechnological applications Enzyme and protein mutations Data analysis and result reporting
Course Precondition
Biochemistry I and II: Basic knowledge of enzymes, protein structures, metabolic pathways, and biochemical reactions. Molecular Biology: Knowledge of PCR, genetic analyses, and DNA/RNA isolation techniques. Chemistry and Physical Chemistry: Understanding of chemical reactions and physical chemistry principles.
Resources
Practice, lehninger and lippincot
Notes
Berg, J. M., Tymoczko, J. L., & Gatto, G. J. (2015). Biochemistry (8th ed.). W.H. Freeman and Company.Walker, J. M. (2009). Molecular Biology and Biotechnology: A Comprehensive Desk Reference (2nd ed.). Wiley.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | To be able to perform evaluate lab analysis |
| LO02 | Apply advanced laboratory techniques: |
| LO03 | Conduct enzymatic analyses: |
| LO04 | Implement PCR and genetic analysis techniques |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Explain the structure and function of the basic tissues of the body. | 3 |
| PLO02 | Belirsiz | Explain the structure and functions of the cell, their physiological mechanisms and bioelectrical events. | 4 |
| PLO03 | Belirsiz | Explain the structure and functions of biochemical molecules involved in metabolism. | 2 |
| PLO04 | Belirsiz | Know the structure and function of genetic material (DNA and RNA), gene expression, genome structure, nucleic acid metabolism and the use of DNA in medicine, the basic principles of heredity. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Laboratory Safety and Ethical Guidelines | Laboratory safety measures, biological and chemical hazards, ethical guidelines, and data integrity. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Proteomics and Protein Isolation | Protein isolation and purification, HPLC, SDS-PAGE, and other protein analysis techniques. | Öğretim Yöntemleri: Tartışma, Alıştırma ve Uygulama |
| 3 | Spectroscopic Methods: UV-Vis, FTIR, and NMR | Structural analysis of biological samples and chemical analysis using UV-Vis, FTIR, and NMR spectroscopy. | Öğretim Yöntemleri: Tartışma, Anlatım |
| 4 | PCR and Real-Time PCR Techniques | Principles of PCR, RT-PCR, and qPCR, gene expression analysis, and amplification techniques. | Öğretim Yöntemleri: Tartışma, Benzetim |
| 5 | Microarray and Genomic Analyses | Gene expression profiling, microarray technology, the use of bioinformatics tools, and data analysis. | Öğretim Yöntemleri: Tartışma, Gösteri |
| 6 | Mass Spectrometry and Proteomics Analysis | Protein structure determination using mass spectrometry, peptide and protein mass analysis, protein-ligand interactions. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 7 | Enzyme Kinetics and Enzymatic Reactions | Michaelis-Menten model, enzyme kinetics, enzyme inhibition, and analysis of substrate binding properties. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Molecular Cloning and Genetic Modification Techniques | Genetic engineering applications: restriction enzymes, ligation, PCR, and gene cloning methods. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 10 | Cellular Imaging and Microscopy Techniques in Biological Systems | Fluorescence microscopy, confocal microscopy, and high-resolution cellular imaging. | Öğretim Yöntemleri: Benzetim, Beyin Fırtınası |
| 11 | Genetic Modification in Biotechnological Applications | Transgenic organisms, CRISPR-Cas9 technology, and genetic modification techniques used in biotechnological product production. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 12 | Enzyme and Protein Mutations | Mutagenesis techniques for functional analysis of enzymes and proteins, protein engineering, and applications. | Öğretim Yöntemleri: Soru-Cevap, Anlatım |
| 13 | Data Analysis and Reporting Results | Laboratory data analysis, statistical tests, modeling, result reporting, and scientific writing. | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 14 | Advanced Protein Structural Analysis and Modifications | Protein structural analysis using X-ray crystallography, NMR, and cryo-EM techniques. | Öğretim Yöntemleri: Tartışma, Anlatım |
| 15 | Biotechnological Product Production and Quality Control | Production, purification, quality control procedures, and techniques used in the biotechnology industry for product manufacturing. | Öğretim Yöntemleri: Gösteri, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav |
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| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 5 | 70 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 182 | ||
| Total Workload / 25 (h) | 7,28 | ||
| ECTS | 7 ECTS | ||