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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT576 |
| Name | Introduction to Basic Molecular Biology Techniques-II |
| Term | 2026-2027 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET BERTAN YILMAZ |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Gene Cloning, DNA Sequence Analysis, DNA Sequence Analysis, Next-Generation Sequencing, Blotting Techniques Northern Blot, Southern Blot, Western Blot Techniques, Understanding Application Areas
Course Content
Gene Cloning, DNA Sequence Analysis, DNA Sequence Analysis, Next-Generation Sequencing, Blotting Techniques Northern Blot, Southern Blot, Western Blot Techniques
Course Precondition
None
Resources
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Notes
Temel ve İleri Moleküler Biyoloji Yöntemleri: Genomik ve Proteomik Analizler, Nobel Tıp Kitabevleri
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Has knowledge of basic molecular biology techniques |
| LO02 | Knows in which researches the basic methods in molecular biology are used |
| LO03 | Has knowledge about the laboratory conditions under which molecular biology applications can be performed |
| LO04 | Comprehends the scientific importance of molecular biological methods |
| LO05 | Knows current molecular biological approaches and has knowledge about literature review |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on undergraduate level qualifications, they develop their knowledge at the level of expertise in the same or a different field. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 4 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | |
| PLO06 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO07 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | 5 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Gene cloning | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 2 | DNA Sequencing, Maxam-Gilbert Method | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 3 | DNA Sequencing, Sanger Method | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 4 | Next-generation sequencing | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 5 | Blotting Techniques | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 6 | Blotting Techniques | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Northern Blot | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Southern Blot | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 10 | Western Blot - Protein Isolation and Quantification | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 11 | Western Blot - Gel Preparation | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 12 | Western Blot - Transfer | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 13 | Western Blot - Antibody Selection and Treatment | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 14 | Western Blot - Imaging | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 15 | Western Blot Application | Laboratory application | Öğretim Yöntemleri: Gösterip Yaptırma |
| 16 | Term Exams | Ölçme Yöntemleri: Ödev |
|
| 17 | Term Exams | Ölçme Yöntemleri: Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||