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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (PhD) (INTERDISCIPLINARY) | |
| Code | BT601 |
| Name | Animal Cell Culture Applications-II |
| Term | 2026-2027 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET BERTAN YILMAZ |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To introduce cell and tissue culture, laboratory requirements, all relevant techniques, conditions, production, and storage.
Course Content
To have a comprehensive understanding of primary cell and tissue culture and be able to explain it in detail. To identify and design the requirements of a cell culture laboratory, list and implement all relevant techniques and conditions, and cultivate and store cells in culture.
Course Precondition
None
Resources
Freshney RI., Culture of Animas Cells:A Manual of Basic Technique
Notes
Freshney RI., Culture of Animas Cells:A Manual of Basic Technique
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Has knowledge of advanced techniques in cell culture |
| LO02 | Knows which cell type to use in a scientific study |
| LO03 | Knows which method to use in cell culture in a scientific study |
| LO04 | Has knowledge of designing cell culture laboratory applications according to a project |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops current and advanced knowledge in the field at the level of expertise with original thought and/or research, based on graduate qualifications. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | 5 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | 5 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | 4 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 4 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | 4 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Primary Cell and Cell Line Culture | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 2 | Adherent and suspension cells | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 3 | Trypsinization | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 4 | Cell Proliferation and Passaging | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 5 | Cell Counting Methods | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 6 | Cell Line Maintenance and Freezing | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 7 | Current cell culture studies screening | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Cell banking | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 10 | Applications of cell lines | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 11 | Cell immortalization | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 12 | Cell transfection | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 13 | Characterization of cells and their products | lecture notes, PowerPoint presentation | Öğretim Yöntemleri: Anlatım |
| 14 | Characterization of cells and their products | Laboratory application | Öğretim Yöntemleri: Gösterip Yaptırma |
| 15 | Current cell culture studies screening 2 | Literature browsing and reading | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Ödev |
|
| 17 | Term Exams | Ölçme Yöntemleri: Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||