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Information
| Unit | |
| Code | SD0839 |
| Name | Chromatographic Techniques |
| Term | 2026-2027 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Üniversite Dersi |
| Label | UCC University Common Course |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. BAHAR MERYEMOĞLU |
| Course Instructor |
Doç. BAHAR MERYEMOĞLU
(Güz)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The objective of this course is to teach the basic logic and working principles of chromatographic separation techniques, which are of critical importance in many areas of daily and professional life such as forensics, food safety, the pharmaceutical industry, environmental analysis, and doping tests, at a popular science level. The course aims to provide students with general scientific literacy and a vision toward analytical techniques through real-life application examples, free from complex formulas.
Course Content
This course covers the basic logic of chromatographic separation methods and the working principles of Gas Chromatography (GC) and Liquid Chromatography (HPLC). Without diving into complex formulas, the practical significance of chromatography is examined at a popular science level through real-world applications in forensics, food safety (adulteration detection), doping control, and the pharmaceutical industry.
Course Precondition
Resources
Enstrümantal Analiz, D. A. Skoog, F. J. Holler, S. R. Crouch, (Çeviri Editörleri: E. Kılıç, F. Köseoğlu, H. Yılmaz), Bilim Yayıncılık, Ankara. Chemical Separations: Principles, Techniques, and Experiments, Clifton E. Meloan, John Wiley & Sons, Inc.
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Evaluates the importance of chromatographic analysis in forensics, food safety, and the pharmaceutical industry |
| LO02 | Explains the fundamental logic and principles of chromatographic separation methods. |
| LO03 | Defines the working principles of gas chromatography (GC) and liquid chromatography (HPLC) at a general level. |
| LO04 | Describes chromatography applications in food adulteration and sports anti-doping testing with concrete examples |
| LO05 | Interprets chromatographic data at a popular science literacy level. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to separation techniques and their importance in daily life. | Conducting general research on separation methods used in daily life (filtration, distillation, etc.). | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Basic concepts of chromatography: How do mixtures separate? | Reviewing the fundamental differences between mixtures and pure substances | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Chemistry at home: Paper and thin-layer chromatography using household items (Simple applications). | Watching simple video demonstrations of paper chromatography experiments using household items like filter paper and colored pens. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | General classification and principles of chromatographic methods. | Brief reading on the concepts of "mobile phase" and "stationary phase" in chromatography. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | : Introduction to Gas Chromatography (GC): Working mechanisms and general design. | Reviewing the general schematic and components of a Gas Chromatography (GC) instrument. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | : Practical applications of GC: Fragrances, essential oils, and flavor analysis in the perfume and cosmetics industry. | Researching how scent and fragrance analyses are conducted in the perfume and cosmetics industry. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | Chromatography in forensics and crime labs: Real-life examples from crime scene investigations | Reviewing popular science articles on the use of chemical analysis in forensic crime scene investigations. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Reviewing lecture notes and fundamental concepts covered in the first 7 weeks. | Ölçme Yöntemleri: Ödev |
| 9 | Introduction to High-Performance Liquid Chromatography (HPLC). | Gaining general information about High-Performance Liquid Chromatography (HPLC) and its everyday applications. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Column and detector types in liquid chromatography: Visual understanding without heavy formulas. | Examining visual illustrations on how chromatography detectors detect compounds. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Anti-doping controls in sports: The critical role of chromatographic testing in global athletics. | Researching the role of laboratory testing in prohibited substance screening by the World Anti-Doping Agency (WADA) | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | Food Safety and Authenticity: Detecting food fraud, adulteration, and counterfeiting (e.g., in honey and olive oil). | Reviewing news articles and case studies regarding food adulteration and authenticity testing in honey and olive oil. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Pharmaceutical applications: Quality control of medications and drug analysis in healthcare. | Gaining a general overview of quality control processes for active pharmaceutical ingredients in medications. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Future trends in separation technologies and general course review. | Exploring innovations in portable analytical devices and modern sensor technologies. | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 15 | Final Exam Preparation and General Review | Summarizing all application areas and chromatographic methods covered throughout the semester. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Preparation for the final exam and comprehensive study of lecture notes. | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation for the final exam / make-up examination. | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 80 | ||
| Total Workload / 25 (h) | 3,20 | ||
| ECTS | 3 ECTS | ||