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Information
| Unit | FACULTY OF SCIENCE AND LETTERS |
| CHEMISTRY PR. | |
| Code | KM334 |
| Name | Biochemistry 2 Lab. |
| Term | 2022-2023 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | C Compulsory |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. RAMAZAN BİLGİN |
| Course Instructor |
Prof. Dr. GÜZİDE YÜCEBİLGİÇ
(Bahar)
(A Group)
(Ins. in Charge)
Prof. Dr. RAMAZAN BİLGİN (Bahar) (A Group) (Asst. Ins.) |
Course Goal / Objective
Application of biochemical analysis methods, evaluation and interpretation of experimental results with theoretical knowledge.
Course Content
Determination of vitamin C in fruit juice, Determination of urea in serum, Isolation of DNA, Determination of Hemoglobin in whole blood, Polarimeter experiment, Determination of molecular weight of proteins with SDS-PAGE electrophoresis, Lowry protein method, Lowry protein method, Immobilization of lipase onto Celite.
Course Precondition
Yok
Resources
Labaoratory text book
Notes
Labaoratory text book
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learn biochemical analysis methods |
| LO02 | Evaluate and interpret experimental results |
| LO03 | Learn isolation of DNA |
| LO04 | Learn determination of molecular weight of proteins with SDS-PAGE electrophoresis |
| LO05 | Learn Lowry protein method |
| LO06 | Learn determination of lipase activity |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Relates his/her knowledge in the field of chemistry to life and industry by relying on his/her knowledge | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Evaluates the developments in the world by following and perceiving the orientations in the industry. | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains awareness of lifelong learning and renews his/her knowledge. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Access to information and associate their knowledge with other fields of science. | 4 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Understands the need to combine information in the application of information and perceives the total quality awareness. | 3 |
| PLO06 | Bilgi - Kuramsal, Olgusal | S/he has knowledge in the use of resources, in the creation of technology depending on the country's resources. | 3 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | S/he conveys what s/he knows to people close or distant to the subject by concretising. | 4 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Gains the ability to access new information and technology. | 5 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Learns to think creatively and produce solutions to problems in the field. | 3 |
| PLO10 | Yetkinlikler - Öğrenme Yetkinliği | To be able to associate information by having the ability of reasoning and transfer what they know to practice. | 5 |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Sets up laboratory equipment and makes decisions alone. | 3 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To be able to follow the current technology in the field of chemistry and gain added value. | 3 |
| PLO13 | Bilgi - Kuramsal, Olgusal | Follows the developments, gains the ability to follow the directions in the field. | 4 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Knowing information sharing and participating in teamwork and directing the team. | 2 |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Gains universal thinking and critical perspective. | 4 |
| PLO16 | Bilgi - Kuramsal, Olgusal | Starts to have a say in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Determination of vitamin C in fruit juice | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Determination of urea in serum | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Isolation of DNA | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Determination of Hemoglobin in whole blood | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Polarimeter experiment | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Lowry protein method | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 10 | Determination of lipase activity by titration | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | Immobilization of lipase onto Celite | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 12 | Immobilization of lipase onto Celite I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | Immobilization of lipase onto Celite II | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 14 | Make-up experiments | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | Make-up experiments I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 16 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 20 |
| General Assessment | ||
| Midterm / Year Total | 100 | 20 |
| 1. Final Exam | - | 80 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 58 | ||
| Total Workload / 25 (h) | 2,32 | ||
| ECTS | 2 ECTS | ||