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Information
| Unit | INSTITUTE OF ADDICTION AND FORENSIC SCIENCES |
| FORENSIC SCIENCES (PhD) (INTERDISCIPLINARY) | |
| Code | ABL606 |
| Name | Forensic Molecular Analysis Methods |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 7 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYŞE SERİN |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Learning of molecular techniques, use of correct technique in forensic case analysis
Course Content
DNA/RNA isolation, quantification, PCR , electrophoresis, RFLP, Blotting, reverse transcription, sequencing methods, realtime PCR
Course Precondition
none
Resources
1.Molecular Forensics, Ralf Rapley, David Whitehouse; 2.Methods in Molecular Biology john Walker
Notes
current artcles
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Understand molecular techniques used for forensic individualization purposes. |
| LO02 | Can use forensic molecular techniques in forensic events. |
| LO03 | Recognizes and uses classical and current technological tools and devices used in molecular studies. |
| LO04 | Knows that technological developments should follow closely. |
| LO05 | Aware of the need to follow up-to-date sources of information. |
| LO06 | Uses molecular techniques and research methods in solving forensic cases and conducting molecular research. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops current and advanced knowledge in forensic sciences at the level of expertise. | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends the research methods related to Forensic Sciences at expertise level. | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical principles for society and person. | 3 |
| PLO04 | Yetkinlikler - Alana Özgü Yetkinlik | Develops a new idea, method, design and/or application on forensic sciences. | 3 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Can write scientific articles related to Forensic Sciences. | 2 |
| PLO06 | Yetkinlikler - Öğrenme Yetkinliği | Are capable of critical analysis, evaluation and synthesis of new and complex ideas. | 2 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Follows evidence-based practices and conducts research on professional practices that will constitute evidence in his field. | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Defends her/his original views when discussing the issues in her field with experts. | 3 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Can communicate with their peers, the larger scholarly community and with society in general about Forensic science area. | 2 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Contributes to the solution of social, scientific, cultural and ethical problems encountered in matters related to forensic sciences. | 2 |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Discusses current developments and her/his own work with different groups in written, oral and visual formats. | 2 |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Uses the knowledge and research methods obtained in the field in solving forensic cases and events. | 3 |
| PLO13 | Bilgi - Kuramsal, Olgusal | Knows the concept of expertise and forensic procedural approach. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Knows crime scene investigation and evidence collection procedures | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Knows the concepts of crime, causes of crime and punishment. | |
| PLO16 | Bilgi - Kuramsal, Olgusal | Recognizes basic forensic medicine and forensic science applications. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction and History | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | RFLP, Southern Blot, Northern blot | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Molecular markers used in forensic genetics | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | DNA/RNA isolation | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 5 | DNA/RNA quantitation | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | End-PCR | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 7 | Real-time PCR | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Sözlü Sınav, Performans Değerlendirmesi |
|
| 9 | Reverse transcription and PCR | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 10 | Reverse transcription, PCR and capillary electrophoresis | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 11 | SNapShot Multiplex test | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 12 | Analysis of mtDNA markers by SNAPSHOT technique | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 13 | Sequence analysis methods and Sanger sequencing technique | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 14 | mtDNA sequencing | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 15 | Overall rating | reading references | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Overal rating_2 | reading references | Ölçme Yöntemleri: Yazılı Sınav, Performans Değerlendirmesi |
| 17 | Term Exams | self-study | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 9 | 9 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 173 | ||
| Total Workload / 25 (h) | 6,92 | ||
| ECTS | 7 ECTS | ||